Post-combustion CO2 capture strategy asks for novel effective processes that can avoid the use of polluting solvents and that can be coupled with CO2 utilization processes. Recent research efforts are focused on the development of CO2 absorption processes promoted by the enzyme carbonic anhydrase [1]. This ubiquitous enzyme is able to catalyze the CO2 hydration reaction so it can be used to enhance CO2 absorption rate into aqueous solvents such as bicarbonate/carbonate solutions. The adoption of the biomimetic strategy for post-combustion CO2 capture is based on the use of environmental friendly solvent (enzyme aqueous solution). Moreover, it provides bicarbonate enriched solutions as intermediate product, such product might be processed in order to convert the captured carbon through further (enzymatic, microbial, photochemical, ...) processes in aqueous phase. The present contribution reports on recent study on the development of carbonic anhydrase biocatalysts through different immobilization techniques. The comparison was made between techniques based on enzyme attachment on solid particles and carrier free immobilization techniques. In both cases the use of paramagnetic solids was included in the development of the biocatalysts, so that magnetic field assisted separation technology may be considered for confinement/recovery of the biocatalyst in/from the reaction volume. The overview of experimental results highlighted advantages and drawbacks of two different CA attachment on activated paramagnetic nanoparticles and of immobilization through Cross Linked Enzyme Aggregates (CLEA) of CA prepared with inclusion of paramagnetic nanoparticles.
Screening of enzyme immobilization techniques for carbonic anhydrase based biocatalyst
Russo ME;Capasso C;
2017-01-01
Abstract
Post-combustion CO2 capture strategy asks for novel effective processes that can avoid the use of polluting solvents and that can be coupled with CO2 utilization processes. Recent research efforts are focused on the development of CO2 absorption processes promoted by the enzyme carbonic anhydrase [1]. This ubiquitous enzyme is able to catalyze the CO2 hydration reaction so it can be used to enhance CO2 absorption rate into aqueous solvents such as bicarbonate/carbonate solutions. The adoption of the biomimetic strategy for post-combustion CO2 capture is based on the use of environmental friendly solvent (enzyme aqueous solution). Moreover, it provides bicarbonate enriched solutions as intermediate product, such product might be processed in order to convert the captured carbon through further (enzymatic, microbial, photochemical, ...) processes in aqueous phase. The present contribution reports on recent study on the development of carbonic anhydrase biocatalysts through different immobilization techniques. The comparison was made between techniques based on enzyme attachment on solid particles and carrier free immobilization techniques. In both cases the use of paramagnetic solids was included in the development of the biocatalysts, so that magnetic field assisted separation technology may be considered for confinement/recovery of the biocatalyst in/from the reaction volume. The overview of experimental results highlighted advantages and drawbacks of two different CA attachment on activated paramagnetic nanoparticles and of immobilization through Cross Linked Enzyme Aggregates (CLEA) of CA prepared with inclusion of paramagnetic nanoparticles.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
