The aim of this work was to identify the transport properties of phase-transfer reaction components through amultiphaseenzyme-loadedmembranesystem. The multiphasesystem is of interest for the preparation of a two-separate phase enzymemembrane reactor (TSP-EMR) applied for the enantioselective conversion of racemic ester of naproxen into the corresponding S-naproxenacid. In this work, the transport properties of the system have been measured in absence of biochemical reaction. Therefore, the overall masstransfer coefficients of reactant (naproxenester) and product (S-naproxenacid) through the two-separate phase membranesystem have been measured using deactivated enzyme-loadedmembrane. The multiphasesystem of interest is composed of an organic and an aqueous phase separated by a polymeric membrane containing the immobilized enzyme. The enzyme was immobilized alone or in presence of oil/water emulsion, in order to either improve enzyme distribution at the o/w interface than transport of reaction components through the membrane. The masstransfer rate through the membrane as a function of axial velocity was evaluated. The transport performance through the membrane itself, the membraneloaded with enzyme, and the membraneloaded with enzyme in presence of emulsion was studied.
Study of mass transfer performance of naproxen acid and ester through a multiphase enzyme-loaded membrane system
Giorno L;Drioli E
2006
Abstract
The aim of this work was to identify the transport properties of phase-transfer reaction components through amultiphaseenzyme-loadedmembranesystem. The multiphasesystem is of interest for the preparation of a two-separate phase enzymemembrane reactor (TSP-EMR) applied for the enantioselective conversion of racemic ester of naproxen into the corresponding S-naproxenacid. In this work, the transport properties of the system have been measured in absence of biochemical reaction. Therefore, the overall masstransfer coefficients of reactant (naproxenester) and product (S-naproxenacid) through the two-separate phase membranesystem have been measured using deactivated enzyme-loadedmembrane. The multiphasesystem of interest is composed of an organic and an aqueous phase separated by a polymeric membrane containing the immobilized enzyme. The enzyme was immobilized alone or in presence of oil/water emulsion, in order to either improve enzyme distribution at the o/w interface than transport of reaction components through the membrane. The masstransfer rate through the membrane as a function of axial velocity was evaluated. The transport performance through the membrane itself, the membraneloaded with enzyme, and the membraneloaded with enzyme in presence of emulsion was studied.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
