Histone post-translational modifications (HPTMs) are recognized as playing crucial roles in the regulation of plant reproduction. In particular, a RPD3/HDA1-class histone deacetylase Athda19 mutant was reported to induce several defects in Arabidopsis such as reduced fertility, shorter siliques, and aborted seeds. The main goal of our work is to functionally characterize SlHDA19 in tomato (Solanum lycopersicum), an important crop and model species for fruit biology. Artificial miRNA approach was used to down-regulate SlHDA19 (Solyc09g091440) in tomato cv. Ailsa Craig. Remarkably, two independent transgenic lines, Slhda19-2 and Slhda19-6, show an early ripening phenotype associated with an increase in the ethylene production. Moreover, these lines display smaller fruits and an early accumulation of the principal carotenoids. Fruit from both lines display a predominance of undeveloped seeds, as has been shown in Arabidopsis siliques, indicated that seed set is also compromised. Currently, cyto-histological analysis of ovules/seeds at different stages postanthesis to characterize embryo development in Slhda19 is ongoing
Down-regulation of histone deacetylase SlHDA19 affects fruit ripening and seed development in tomato
Coniglio A;Termolino P;Cremona G;Paparo R;Conicella C;Consiglio MF
2017
Abstract
Histone post-translational modifications (HPTMs) are recognized as playing crucial roles in the regulation of plant reproduction. In particular, a RPD3/HDA1-class histone deacetylase Athda19 mutant was reported to induce several defects in Arabidopsis such as reduced fertility, shorter siliques, and aborted seeds. The main goal of our work is to functionally characterize SlHDA19 in tomato (Solanum lycopersicum), an important crop and model species for fruit biology. Artificial miRNA approach was used to down-regulate SlHDA19 (Solyc09g091440) in tomato cv. Ailsa Craig. Remarkably, two independent transgenic lines, Slhda19-2 and Slhda19-6, show an early ripening phenotype associated with an increase in the ethylene production. Moreover, these lines display smaller fruits and an early accumulation of the principal carotenoids. Fruit from both lines display a predominance of undeveloped seeds, as has been shown in Arabidopsis siliques, indicated that seed set is also compromised. Currently, cyto-histological analysis of ovules/seeds at different stages postanthesis to characterize embryo development in Slhda19 is ongoingI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
