Peripheral blood lymphocytes were cultured and treated for early- and late-BrdU incorporation to perform replicating G- and R-banding patterns, respectively. Slides were treated for GBG-, RBA- and RBG-banding techniques. Improved banded karyotypes at early- (350 bands) and pro-metaphase (500 bands) stage were performed and GBG- and RBA-banded prometaphase karyotypes were presented for the first time on this species. All chromosomes, including the small acrocentrics, show clear and distinguishable G- and R-banding patterns. Chromosome identification followed the latest chromosome standard nomenclature (ISCNH 1997). This study is also our contribution to further standard karyotype attempts at the prometaphase stage.
An improved characterization of horse (Equus caballus, 2n = 64) chromosomes by using replicating G and R banding patterns
Iannuzzi L;Di Meo GP;Perucatti A;Incarnato D;
2003
Abstract
Peripheral blood lymphocytes were cultured and treated for early- and late-BrdU incorporation to perform replicating G- and R-banding patterns, respectively. Slides were treated for GBG-, RBA- and RBG-banding techniques. Improved banded karyotypes at early- (350 bands) and pro-metaphase (500 bands) stage were performed and GBG- and RBA-banded prometaphase karyotypes were presented for the first time on this species. All chromosomes, including the small acrocentrics, show clear and distinguishable G- and R-banding patterns. Chromosome identification followed the latest chromosome standard nomenclature (ISCNH 1997). This study is also our contribution to further standard karyotype attempts at the prometaphase stage.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
