Innovative and Sustainable Production of Biopolymers

This work aims to develop methodologies and tools to support the design and management of sustainable processes for the production of biodegradable polyhydroxyalcanoates (PHAs) biopolymers. PHAs are linear polyesters produced in nature by bacteria through aerobic fermentation of many carbon sources, completely biodegradable and biocompatible. We carried out a study inherent to the advancement of an innovative, cost-effective and environmentally sustainable technology for isolating PHAs from bacteria mixed cultures by combining: (a) innovative cells' pre-treatments and polymer purification's strategy by means of TiO2/UV or Ag0 nanostructured materials; (b) polymer extraction through a green and safe system directly applicable to bacterial cultures, which combines the advantages of solvent extraction and these of dissolution of the non-PHAs cellular matrix through surfactants; (c)monitoring and control tools for process energy and efficiencymanagement. The outcomes put the basis for the design and subsequent building of a working pilot system for the production of completely biodegradable and biocompatible PHAs.The efficiency can be improved and the investments and operating costs can be decreased thanks to the optimization of the production process with the introduction of safe and cheap PHAs extraction route without use of toxic and harmful chemicals and the integration of monitoring and automation tools. The engineering and integration of nano-TiO2 phase within textile fibres and their use as photocatalytic active media for bacteria pre- and post-treatment of waste water added a new opportunity for improving process efficiency and sustainability.