EIS-based biosensors in foodborne pathogen detection with a special focus on Listeria monocytogenes

This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens.

In this chapter methods and protocols for surfaces adapted to electrochemical impedance detection, antibody binding, electrolyte couples used, and instrumentation for EIS binsensing are presented. Various technical bottlenecks have been overcome in recent years. Other limitations still present in this technique are discussed. We present the most recent applications in food pathogens detection, either with EIS methods as well with other antibody-based platforms.