Ghrelin (Ghre), a gut-brain peptide hormone, and its receptor (GHS-R 1A) are expressed in different part of the central nervous system. Recently, several studies describe this peptide as a neuroprotective factor showing antioxidant, anti-inflammatory and anti-apoptotic properties. Olfactory Ensheathing Cells (OECs) are particular glial cells responsible for the continuous neurogenesis in the mammalian olfactory system. Studies on axonal regeneration in animals with a spinal cord damage have showed that OEC transplantations may restore the injury. Furthermore, the OECs are able to secrete a variety of neurotrophic factors, promote axonal growth and support the remyelination of damaged axons. The aim of this in vitro study was to investigate on the effect of Ghrelin on the OECs. Primary OECs were isolated from neonatal rodent olfactory bulbs. Some cells were processed immunocytochemically to the detection of Ghre and its receptor. Other cells were grown with Ghrelin peptide at different concentrations (1?M and 2?M) and times (1-3 and 7 days). To analyse the results, 3-[4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl] tetrazolium bromide test (MTT) and immunocytochemical procedures were used. Our immunocytochemical study highlights, for the first time, that OECs express both Ghre and GHS-R1A and in addition show an OEC characterization towards a neuronal phenotype, as they are Neuregulin and MAP-2 positive. Moreover, the results obtained through the MTT test indicate that Ghre at 2?M after 7 days stimulates cell viability, in fact a significant increase (p<0.05) of OECs was observed. Besides the cells exhibit longer fibers than controls. In conclusion, the presence and the action of Ghre on OECs could open a doorway that leads to investigate more deeply on the world of these cells.
EFFECT OF GHRELIN ON DIFFERENT MARKER EXPRESSION IN OLFACTORY ENSHEATHING CELLS. AN IN VITRO STUDY.
Pellitteri R
2018
Abstract
Ghrelin (Ghre), a gut-brain peptide hormone, and its receptor (GHS-R 1A) are expressed in different part of the central nervous system. Recently, several studies describe this peptide as a neuroprotective factor showing antioxidant, anti-inflammatory and anti-apoptotic properties. Olfactory Ensheathing Cells (OECs) are particular glial cells responsible for the continuous neurogenesis in the mammalian olfactory system. Studies on axonal regeneration in animals with a spinal cord damage have showed that OEC transplantations may restore the injury. Furthermore, the OECs are able to secrete a variety of neurotrophic factors, promote axonal growth and support the remyelination of damaged axons. The aim of this in vitro study was to investigate on the effect of Ghrelin on the OECs. Primary OECs were isolated from neonatal rodent olfactory bulbs. Some cells were processed immunocytochemically to the detection of Ghre and its receptor. Other cells were grown with Ghrelin peptide at different concentrations (1?M and 2?M) and times (1-3 and 7 days). To analyse the results, 3-[4, 5-dimethylthiazol- 2-yl)-2,5-diphenyl] tetrazolium bromide test (MTT) and immunocytochemical procedures were used. Our immunocytochemical study highlights, for the first time, that OECs express both Ghre and GHS-R1A and in addition show an OEC characterization towards a neuronal phenotype, as they are Neuregulin and MAP-2 positive. Moreover, the results obtained through the MTT test indicate that Ghre at 2?M after 7 days stimulates cell viability, in fact a significant increase (p<0.05) of OECs was observed. Besides the cells exhibit longer fibers than controls. In conclusion, the presence and the action of Ghre on OECs could open a doorway that leads to investigate more deeply on the world of these cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
