Background:Mammalian serum albumins (SA) are thermolabileallergens present in milk, meat and animal dander. The sensitizationcan occur through ingestion, inhalation and an IgE co-recognitionmight be detected. Objectives: To explore the IgE sensitization pro-files to SA in a population of 6113 patients. Method:The sera of 6113 patients, average age 33 years, weretested by means of the FABER®multiplex IgE detection assay. Theanalysis was focused on 10 SA (Bos d 6, Equ c 3, Ovi a 6, Ory c 6,Sus s 1, Can f 3, Fel d 2, Mus m 4, Rat n 4, Hom s HSA), 6 milkextracts (Bos d, Bub b, Cam d, Cap h, Equ c, Equ as), 4 cow's milkproteins (Bos d 4, Bos d 5, Bos d 8, Bos d LF), 4 Meat Extracts (Bosd, Ory c, Ovi a, Sus s), 8 epithelium extracts (cat, dog, guinea pig,hamster, horse, mouse, rabbit, rat) and 7 genuine animal allergenicproteins (Can f 1, Can f 2, Can f 5, Fel d 1, Mus m 1, Rat n 1) Results:None of the tested sera was positive for HSA. Out of the6113 patients, 433 (7%) are SA+patients, sensitized to at least oneSA. The distribution by age shows a peak of 180 patients in the firstdecade (41.6%), progressively decreasing with age. Bos d 6 is glob-ally the most recognized, 54% of the SA+, followed by Ovi a 6(46%) and Fel d 2 (45%), with a peak in the first decade of 83% fol-lowed by Ovi a 6 (52%) and Sus m 1 (39%). Within the first decade94% of patients sensitized to Bos d 6 are also sensitized to at leastone milk protein, this prevalence becomes 92% when consideringthe entire SA population. Concerning the other SA, the prevalenceout of the 433 SA+are as follows: Sus s 1 35%, Ory c 6 33%, Equ c3 29%, Mus m 428%, Rat n 4 28%. SA+subjects were more fre-quently positive for milk, meat, and epithelium extracts regardlessreal exposures. Genuine markers of milk sensitization and of epithe-lia were recorded positive in the SA+subjects helping defining theirfull profiles.216| Conclusion:Sensitization to Bos d 6 seems to be driven by milkingestion, both globally and within first decade. Further studies arerequired to detect the real driver of the sensitization to the otherSA. Genuine allergens help in the definition of single patient sensiti-zation profiles. The specific advantage of FABER®relies on thechance of testing patients to a broad panel of proteins and extracts.Interpreting the sensitization profiles with an accurate patient's his-tory could provide improvement to the allergic patient's life.
Different sensitization profiles to mammalian serum albumins as detected by a new IgE detection multiplex assay
Tuppo L;Tamburrini M;Ciardiello M A;
2018
Abstract
Background:Mammalian serum albumins (SA) are thermolabileallergens present in milk, meat and animal dander. The sensitizationcan occur through ingestion, inhalation and an IgE co-recognitionmight be detected. Objectives: To explore the IgE sensitization pro-files to SA in a population of 6113 patients. Method:The sera of 6113 patients, average age 33 years, weretested by means of the FABER®multiplex IgE detection assay. Theanalysis was focused on 10 SA (Bos d 6, Equ c 3, Ovi a 6, Ory c 6,Sus s 1, Can f 3, Fel d 2, Mus m 4, Rat n 4, Hom s HSA), 6 milkextracts (Bos d, Bub b, Cam d, Cap h, Equ c, Equ as), 4 cow's milkproteins (Bos d 4, Bos d 5, Bos d 8, Bos d LF), 4 Meat Extracts (Bosd, Ory c, Ovi a, Sus s), 8 epithelium extracts (cat, dog, guinea pig,hamster, horse, mouse, rabbit, rat) and 7 genuine animal allergenicproteins (Can f 1, Can f 2, Can f 5, Fel d 1, Mus m 1, Rat n 1) Results:None of the tested sera was positive for HSA. Out of the6113 patients, 433 (7%) are SA+patients, sensitized to at least oneSA. The distribution by age shows a peak of 180 patients in the firstdecade (41.6%), progressively decreasing with age. Bos d 6 is glob-ally the most recognized, 54% of the SA+, followed by Ovi a 6(46%) and Fel d 2 (45%), with a peak in the first decade of 83% fol-lowed by Ovi a 6 (52%) and Sus m 1 (39%). Within the first decade94% of patients sensitized to Bos d 6 are also sensitized to at leastone milk protein, this prevalence becomes 92% when consideringthe entire SA population. Concerning the other SA, the prevalenceout of the 433 SA+are as follows: Sus s 1 35%, Ory c 6 33%, Equ c3 29%, Mus m 428%, Rat n 4 28%. SA+subjects were more fre-quently positive for milk, meat, and epithelium extracts regardlessreal exposures. Genuine markers of milk sensitization and of epithe-lia were recorded positive in the SA+subjects helping defining theirfull profiles.216| Conclusion:Sensitization to Bos d 6 seems to be driven by milkingestion, both globally and within first decade. Further studies arerequired to detect the real driver of the sensitization to the otherSA. Genuine allergens help in the definition of single patient sensiti-zation profiles. The specific advantage of FABER®relies on thechance of testing patients to a broad panel of proteins and extracts.Interpreting the sensitization profiles with an accurate patient's his-tory could provide improvement to the allergic patient's life.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
