Methods: Thiol reactivity has been probed in kinetic experiments in order to assess the protein matrix permeation properties towards the bulky thiol reactive DTNB (5,5'-dithiobis-2-nitrobenzoic acid) molecule.
Background: A set of engineered ferritin mutants from Archaeoglobus fulgidus (Af-Ft) and Pyrococcus furiosus (Pf-Ft) bearing cysteine thiols in selected topological positions inside or outside the ferritin shell have been obtained. The two apo-proteins were taken as model systems for ferritin internal cavity accessibility in that Af-Ft is characterized by the presence of a 45 A wide aperture on the protein surface whereas Pf-Ft displays canonical (threefold) channels.
Probing bulky ligand entry in engineered archaeal ferritins
Ruzicka, Barbara;Boffi, Alberto;Falvo, Elisabetta;
2017
Abstract
Background: A set of engineered ferritin mutants from Archaeoglobus fulgidus (Af-Ft) and Pyrococcus furiosus (Pf-Ft) bearing cysteine thiols in selected topological positions inside or outside the ferritin shell have been obtained. The two apo-proteins were taken as model systems for ferritin internal cavity accessibility in that Af-Ft is characterized by the presence of a 45 A wide aperture on the protein surface whereas Pf-Ft displays canonical (threefold) channels.| File | Dimensione | Formato | |
|---|---|---|---|
|
prod_370131-doc_123570.pdf
solo utenti autorizzati
Descrizione: Probing bulky ligand entry in engineered archaeal ferritins
Tipologia:
Versione Editoriale (PDF)
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
698.71 kB
Formato
Adobe PDF
|
698.71 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
