Durant les enquêtes effectuées pour déterminer les nématodes phytoparasites associés à l'olivier en Tunisie, une forte infestation de l'olivier par les nématodes à kystes Heterodera mediterranea a été détectée dans une oliveraie située à Moknine (Monastir, Sahel Tunisien). Une approche taxonomique qui intègre les études morphobiométriques et les analyses moléculaires a été réalisée pour caractériser la population tunisienne de H. mediterranea. Les analyses phylogénétiques basées sur les régions ITS et les segments d'expansion D2-D3 de la grande sous-unité ribosomal 28S de l'ADN ribosomal ont montré que la population tunisienne de H. mediterranea appartient au groupe Schachtii. Ceci est le premier signalement de ce nématode en Tunisie.
A survey was conducted in Tunisia to detect the presence of plant parasitic nematodes associated to olive trees. A high infection of olive roots and soil by the cyst nematode Heterodera mediterranea was detected in olive orchards located in the region of Moknine (Monastir, Sahel of Tunisia). Integrative taxonomic approaches (morphological, morphometrical and molecular analyses) were carried out in order to characterize the Tunisian population of H. mediterranea. Phylogenetic analyses of the ITS region, the D2-D3 expansion segments of the 28S rRNA gene and 18S rRNA gene highly supported that H. mediterranea from Tunisia belongs to the Schachtii group. So far, this is the first report of this nematode in Tunisia.
First Report of the Cyst Nematode Heterodera mediterranea on Olive Trees in Tunisia
Troccoli A;Fanelli E;Radicci V;De Luca F
2018
Abstract
A survey was conducted in Tunisia to detect the presence of plant parasitic nematodes associated to olive trees. A high infection of olive roots and soil by the cyst nematode Heterodera mediterranea was detected in olive orchards located in the region of Moknine (Monastir, Sahel of Tunisia). Integrative taxonomic approaches (morphological, morphometrical and molecular analyses) were carried out in order to characterize the Tunisian population of H. mediterranea. Phylogenetic analyses of the ITS region, the D2-D3 expansion segments of the 28S rRNA gene and 18S rRNA gene highly supported that H. mediterranea from Tunisia belongs to the Schachtii group. So far, this is the first report of this nematode in Tunisia.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.