Upgrade sperm FISH analysis of meiotic segregation in a river buffalo bull carrier a rob(1p;18)

An upgrade by triple-colour fluorescence in situ hybridization (FISH) in River Buffalo sperms was performed on chromosomes involved in the translocation (BBU1 and BBU18) using three different pools of specific bovine BAC (INRA library) probes, mapping in BBU1q, BBU1p and BBU18 (homologous to BTA1, BTA27 and BTA18, respectively). The meiotic segregation pattern was examined in the carrier and in the control counting 2.500 total sperms and 2.500 total motile sperms on both animals. The results revealed different frequencies of normal and chromosomally balanced sperms (alternate group) as follows: 24.1% and 11.4% (total of 35.5%) of total sperms in the carrier, while in the control there were 97.1% and 0.4% (total of 97.8%) respectively; 58.4% and 22.2% (total of 80.6%) of motile sperms in the carrier, while in the control there were93.6% and 3.3% (total of 96.1%) respectively. These data have shown the increase of percentage in alternate group of motile sperms fraction that represents the real sperm population able to fertilize an oocyte. This result revealed a connection between sperm motility and DNA distribution, underling the importance of Sperm-FISH analysis in reproducers.