During spring-summer 2012, 2013, and 2015, 10 samples of bell pepper (Capsicum annuum) plants showing basal bright rings and mosaic of the leaves with some necrotic patches, necrotic stems, and fruits with brown patches and corky rings, were identified in the Campania region (southern Italy), in a family garden (four plants in 2012 and three plants in 2015 of the Friariello ecotype) and in a commercial greenhouse (two plants of the Papaccella ecotype in 2012 and one plant of the Eppo hybrid in 2013). Symptomatic samples were tested at the Institute for Sustainable Plant Protection in Portici (Napoli, Italy). Screening of leaf and fruit symptomatic tissues by double antibody sandwich (DAS)-ELISA with commercial kits of antisera (Bioreba AG, Switzerland) to Cucumber mosaic virus(CMV), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus(ToMV), Pepper mild mottle virus (PMMoV), and by indirect plate trapped antigen (PTA)-ELISA against potyviruses (potygroup test), gave negative results, whereas all samples tested positive in antigen-coated-plate (ACP)-ELISA with antisera, kindly provided by Dr. P. Roggero (Plant Virology Institute, Torino, Italy), produced against a tomato isolate (namely TI1 isolate) of Parietaria mottle virus (PMoV). The results from indirect ELISA were subsequently confirmed with reverse transcription (RT)-PCR using PMoV specific primers designed to amplify the entire gene of the movement protein (MP): PMoVMP1a (5?-AGGGTTTGAAATGGCATTTGTTC-3?) and PMoVMP2b (5?-CTTAGTGGACATTCACGAATATC-3?). A healthy pepper plant was used as a negative control both in ELISA and RT-PCR assays. RT-PCR products of 916 bp, which covered approximately 40.4% of PMoV RNA3 (isolate T32 as reference, accession number KT005245), were obtained in all symptomatic samples and one amplicon for each pepper ecotype and year of sampling was cloned and sequenced. The nucleotide sequences of the MP gene of three Italian PMoV isolates were deposited in GenBank under accession numbers LT160068 (isolate Fri-1, from the Friariello ecotype, in 2012), LT160069 (isolate Pap-1, from the Papaccella ecotype, in 2012), and LT160070 (isolate Fri-2, from the Friariello ecotype, in 2015). Isolates Fri-1 and Pap-1 showed 98.4 and 97.6% identity with the Spanish PMoV isolate JBT1 from tomato (accession number AM182747), respectively, while Fri-2 showed 96.7% identity with the Spanish PMoV isolate AC1 from tomato (accession number AM182743). To our knowledge, this is the first report of PMoV infecting bell pepper in Italy. In 2004, PMoV was identified in pepper crops in Spain (Janssen et al. 2005). The virus is also known to infect economically important plants as tomato in Italy, France, Spain, and Greece (Aramburu 2001;Marchoux et al. 1999; Roggero et al. 2000), as well as the ornamental Mirabilis jalapa (Parrella 2002). This new finding of PMoV in pepper in Italy confirms the tendency of the virus to broaden its range of natural hosts, particularly concerning species of economic interest. The symptoms induced by PMoV on pepper can be visually confused with those caused by other viruses infecting pepper (e.g., TSWV, CMV). Therefore, it is necessary to monitor regularly for the presence of PMoV in pepper crops in order to know the real incidence of the disease in the field with the aim of developing efficient and durable disease control strategies.
Severe symptoms of mosaic and necrosis in bell pepper associated with parietaria mottle virus in Italy
Parrella G;Troiano E
2016
Abstract
During spring-summer 2012, 2013, and 2015, 10 samples of bell pepper (Capsicum annuum) plants showing basal bright rings and mosaic of the leaves with some necrotic patches, necrotic stems, and fruits with brown patches and corky rings, were identified in the Campania region (southern Italy), in a family garden (four plants in 2012 and three plants in 2015 of the Friariello ecotype) and in a commercial greenhouse (two plants of the Papaccella ecotype in 2012 and one plant of the Eppo hybrid in 2013). Symptomatic samples were tested at the Institute for Sustainable Plant Protection in Portici (Napoli, Italy). Screening of leaf and fruit symptomatic tissues by double antibody sandwich (DAS)-ELISA with commercial kits of antisera (Bioreba AG, Switzerland) to Cucumber mosaic virus(CMV), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV), Tomato mosaic virus(ToMV), Pepper mild mottle virus (PMMoV), and by indirect plate trapped antigen (PTA)-ELISA against potyviruses (potygroup test), gave negative results, whereas all samples tested positive in antigen-coated-plate (ACP)-ELISA with antisera, kindly provided by Dr. P. Roggero (Plant Virology Institute, Torino, Italy), produced against a tomato isolate (namely TI1 isolate) of Parietaria mottle virus (PMoV). The results from indirect ELISA were subsequently confirmed with reverse transcription (RT)-PCR using PMoV specific primers designed to amplify the entire gene of the movement protein (MP): PMoVMP1a (5?-AGGGTTTGAAATGGCATTTGTTC-3?) and PMoVMP2b (5?-CTTAGTGGACATTCACGAATATC-3?). A healthy pepper plant was used as a negative control both in ELISA and RT-PCR assays. RT-PCR products of 916 bp, which covered approximately 40.4% of PMoV RNA3 (isolate T32 as reference, accession number KT005245), were obtained in all symptomatic samples and one amplicon for each pepper ecotype and year of sampling was cloned and sequenced. The nucleotide sequences of the MP gene of three Italian PMoV isolates were deposited in GenBank under accession numbers LT160068 (isolate Fri-1, from the Friariello ecotype, in 2012), LT160069 (isolate Pap-1, from the Papaccella ecotype, in 2012), and LT160070 (isolate Fri-2, from the Friariello ecotype, in 2015). Isolates Fri-1 and Pap-1 showed 98.4 and 97.6% identity with the Spanish PMoV isolate JBT1 from tomato (accession number AM182747), respectively, while Fri-2 showed 96.7% identity with the Spanish PMoV isolate AC1 from tomato (accession number AM182743). To our knowledge, this is the first report of PMoV infecting bell pepper in Italy. In 2004, PMoV was identified in pepper crops in Spain (Janssen et al. 2005). The virus is also known to infect economically important plants as tomato in Italy, France, Spain, and Greece (Aramburu 2001;Marchoux et al. 1999; Roggero et al. 2000), as well as the ornamental Mirabilis jalapa (Parrella 2002). This new finding of PMoV in pepper in Italy confirms the tendency of the virus to broaden its range of natural hosts, particularly concerning species of economic interest. The symptoms induced by PMoV on pepper can be visually confused with those caused by other viruses infecting pepper (e.g., TSWV, CMV). Therefore, it is necessary to monitor regularly for the presence of PMoV in pepper crops in order to know the real incidence of the disease in the field with the aim of developing efficient and durable disease control strategies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
