Aim: To investigate the molecular effects of 30s treatment with a Blue LED device on a murine model ulcer. Method: 63 CD1 mice were anesthetized and randomized in three groups: GI, GII, GIII. In GI and GII groups one full-thickness wound on the shaved back was performed with a 4mm large punch, while in GIII group two wounds were induced. The animals of the GI group and only one lesion of GIII group underwent a 30s treatment with a Blue LED light, while the GII group were left to naturally recover. The animals were sacrificed after 1, 3, 6, 9, 24 hours and 7 and 14 days after the treatment and skin biopsies from the back were obtained. During all the treatments, the superficial skin temperature was monitored with a thermal camera. A customized ELISA kit enabled to study EGF, bFGF, VEGF, TNF-?, MMP-2 and PRO-MMP-9 at different postoperative time points; moreover, a histological analysis with hematoxylin-eosin method was performed. Results / Discussion: The expression of TNF-alpha, bFGF and Pro-MMP-9 evidenced a modulation due to the light treatment. The GIII group shows differences in the healing process between the treated and untreated wound. Conclusion: These experimental results are supporting our previous studies: the Blue LED light treatment is a modulator of the wound healing process. Its application can be extremely useful in the treatment of ulcers and chronic wounds.
BLUE LED light effects in an in vivo murine model of ulcer
Francesca Rossi;Giada Magni;Francesca Tatini;Roberto Pini
2019
Abstract
Aim: To investigate the molecular effects of 30s treatment with a Blue LED device on a murine model ulcer. Method: 63 CD1 mice were anesthetized and randomized in three groups: GI, GII, GIII. In GI and GII groups one full-thickness wound on the shaved back was performed with a 4mm large punch, while in GIII group two wounds were induced. The animals of the GI group and only one lesion of GIII group underwent a 30s treatment with a Blue LED light, while the GII group were left to naturally recover. The animals were sacrificed after 1, 3, 6, 9, 24 hours and 7 and 14 days after the treatment and skin biopsies from the back were obtained. During all the treatments, the superficial skin temperature was monitored with a thermal camera. A customized ELISA kit enabled to study EGF, bFGF, VEGF, TNF-?, MMP-2 and PRO-MMP-9 at different postoperative time points; moreover, a histological analysis with hematoxylin-eosin method was performed. Results / Discussion: The expression of TNF-alpha, bFGF and Pro-MMP-9 evidenced a modulation due to the light treatment. The GIII group shows differences in the healing process between the treated and untreated wound. Conclusion: These experimental results are supporting our previous studies: the Blue LED light treatment is a modulator of the wound healing process. Its application can be extremely useful in the treatment of ulcers and chronic wounds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
