An in vitro study on keloid fibroblasts treated with Blue LED light device Aim: We want to investigate when human keloid fibroblasts are influenced by Blue LED light. We use a LED-device that emitting in a range of 410-430 nm at 235 mW . Method: We used six keloids and three samples of healthy skin. Primary cultures of human keloid fibroblasts (HKF) were established. We used confocal microscopy to characterize the cultured cells and two colourimetric tests (Cell Counting Kit 8 and Sulforhodamine B based) to study the cell metabolism and viability. We conducted 96 tests after 24 hours and 68 tests at 48 hours after the treatment on all samples and each experiment was performed in triplicate. We also used Raman spectroscopy to study the direct effects of Blue LED light on cytochrome C, and electrophysiological recording to analyse the fibroblasts membrane currents during the irradiation with the Blue LED light in order to investigate about the effects on cell cycle. Results / Discussion: after 24 hours from the treatment, fibroblasts metabolism decrease at the increases of the irradiation time while cell viability unchanged. At 48 hours, metabolism shows the same behaviour but also cell viability decrease at the increases of the irradiation time. From electrophysiological recording, we demonstrated that the Blue LED light has an effect on keloid fibroblasts after three minutes from the treatment. Conclusion: we pointed out that the Blue LED light has a direct effect on keloid fibroblasts. This kind of treatment could be consider an innovative and non-invasive approach in the management about several types of skin lesions.
An in vitro study on keloid fibroblasts irradiated by a Blue LED light device
G Magni;F Rossi;M Banchelli;P Matteini;R Pini;
2019
Abstract
An in vitro study on keloid fibroblasts treated with Blue LED light device Aim: We want to investigate when human keloid fibroblasts are influenced by Blue LED light. We use a LED-device that emitting in a range of 410-430 nm at 235 mW . Method: We used six keloids and three samples of healthy skin. Primary cultures of human keloid fibroblasts (HKF) were established. We used confocal microscopy to characterize the cultured cells and two colourimetric tests (Cell Counting Kit 8 and Sulforhodamine B based) to study the cell metabolism and viability. We conducted 96 tests after 24 hours and 68 tests at 48 hours after the treatment on all samples and each experiment was performed in triplicate. We also used Raman spectroscopy to study the direct effects of Blue LED light on cytochrome C, and electrophysiological recording to analyse the fibroblasts membrane currents during the irradiation with the Blue LED light in order to investigate about the effects on cell cycle. Results / Discussion: after 24 hours from the treatment, fibroblasts metabolism decrease at the increases of the irradiation time while cell viability unchanged. At 48 hours, metabolism shows the same behaviour but also cell viability decrease at the increases of the irradiation time. From electrophysiological recording, we demonstrated that the Blue LED light has an effect on keloid fibroblasts after three minutes from the treatment. Conclusion: we pointed out that the Blue LED light has a direct effect on keloid fibroblasts. This kind of treatment could be consider an innovative and non-invasive approach in the management about several types of skin lesions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
