The involvement of the immunodominant membrane protein Imp of 16SrV-D phytoplasma in the interaction with insect proteins was investigated. The His-tagged partial Imp protein and a rabbit polyclonal antibody were produced and used in Far-Western dot Blot (FWdB) assays. Total native proteins were extracted from batches of six insect species (Scaphoideus titanus, Euscelidius variegatus, Macrosteles quadripunctulatus, Ricania speculum, Metcalfa pruinosa and Zyginidia pullula). FWdB showed interaction of Imp fusion protein with total proteins of S. titanus, E. variegatus (known as efficient vectors) and M. quadripuncltulatus (potential vector), while no interaction signal was detected with the other three species that are reported as non-phytoplasma vectors.
Role of membrane protein Imp of "flavescence dorée" phytoplasma in interaction with insect proteins: preliminary results
Rossi M;Galetto L
2019
Abstract
The involvement of the immunodominant membrane protein Imp of 16SrV-D phytoplasma in the interaction with insect proteins was investigated. The His-tagged partial Imp protein and a rabbit polyclonal antibody were produced and used in Far-Western dot Blot (FWdB) assays. Total native proteins were extracted from batches of six insect species (Scaphoideus titanus, Euscelidius variegatus, Macrosteles quadripunctulatus, Ricania speculum, Metcalfa pruinosa and Zyginidia pullula). FWdB showed interaction of Imp fusion protein with total proteins of S. titanus, E. variegatus (known as efficient vectors) and M. quadripuncltulatus (potential vector), while no interaction signal was detected with the other three species that are reported as non-phytoplasma vectors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
