Jujube (Ziziphus jujuba Mill) trees are widely planted in temperate zones of China, but it also grows in Europe and Australia. Jujube trees affected by a novel disease, causing yellow mottle mosaic on the leaves and fruit distortion, were found in China (Liaoning province). The disease was named jujube yellow mottle disease (JYMD) and a novel negative-stranded (ns) RNA virus was identified in diseased plant by high-throughput sequencing approaches. We propose to tentatively name this novel virus jujube yellow mottle-associated virus (JYMaV) because a preliminary survey, performed using a specific RT-PCR detection method, showed the close association of the virus with JYMD. Virus-like particles and double-membrane-bound bodies were identified in infected tissues by Eeectron microscopy, showing that JYMaV is an enveloped virus. Its genome i s composed by at least six segments, with each segment containing in the negative polarity a single open reading frame flanked by terminal untranslated regions with conserved and complementary terminal ends. The putative proteins encoded by RNA1 (7.1 kb), RNA2 (2.2 kb) and RNA3 (1.2 kb), based on their conserved motifs, have been identified as the RNA dependent RNA polymerase, the glycoprotein and the nucleocapsid proteins, respectively. All these proteins share significant sequence identity (52.1-70,4%) with the corresponding proteins of raspberry leaf blotch virus (RLBV), the type member of genus Emaravirus (family Fimoviridae). RNA4 (1.5 kb) and RNA5 (1.2 kb), share high sequence identity with each other and code for two putative proteins also related to a protein involved in RBLV movement within the host. In contrast, the protein encoded by JYMaV RNA6 does not share significant sequence identity with previously reported proteins and no motif suggesting a possible functiona l role was identified. Phylogenetic analyses strongly support close relationships of JYMaV with RLBV and the other members of the genus Emaravirus. Based on these data we propose to classify JYMaV as the representative member of a novel species in the genus Emaravirus.
A novel emaravirus from Jujube trees
Di Serio F;
2019
Abstract
Jujube (Ziziphus jujuba Mill) trees are widely planted in temperate zones of China, but it also grows in Europe and Australia. Jujube trees affected by a novel disease, causing yellow mottle mosaic on the leaves and fruit distortion, were found in China (Liaoning province). The disease was named jujube yellow mottle disease (JYMD) and a novel negative-stranded (ns) RNA virus was identified in diseased plant by high-throughput sequencing approaches. We propose to tentatively name this novel virus jujube yellow mottle-associated virus (JYMaV) because a preliminary survey, performed using a specific RT-PCR detection method, showed the close association of the virus with JYMD. Virus-like particles and double-membrane-bound bodies were identified in infected tissues by Eeectron microscopy, showing that JYMaV is an enveloped virus. Its genome i s composed by at least six segments, with each segment containing in the negative polarity a single open reading frame flanked by terminal untranslated regions with conserved and complementary terminal ends. The putative proteins encoded by RNA1 (7.1 kb), RNA2 (2.2 kb) and RNA3 (1.2 kb), based on their conserved motifs, have been identified as the RNA dependent RNA polymerase, the glycoprotein and the nucleocapsid proteins, respectively. All these proteins share significant sequence identity (52.1-70,4%) with the corresponding proteins of raspberry leaf blotch virus (RLBV), the type member of genus Emaravirus (family Fimoviridae). RNA4 (1.5 kb) and RNA5 (1.2 kb), share high sequence identity with each other and code for two putative proteins also related to a protein involved in RBLV movement within the host. In contrast, the protein encoded by JYMaV RNA6 does not share significant sequence identity with previously reported proteins and no motif suggesting a possible functiona l role was identified. Phylogenetic analyses strongly support close relationships of JYMaV with RLBV and the other members of the genus Emaravirus. Based on these data we propose to classify JYMaV as the representative member of a novel species in the genus Emaravirus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
