The control of grapevine trunk diseases (GTDs) represents a big challenge for grapevine nurseries due to the high infection risks on the propagating material. Plant material disinfection before storage and the grafting practice are both potential factors able to affect the future GTDs development in young vines in field. In this framework, this research is aimed to evaluate the microbiome (especially taking into account GTDs pathogens) in the propagation material undergone through different disinfection treatments, and to compare the impact of three grafting methods on the formation of an active and functional vascular system. To assess the first aim, the microbiome of the scion (cv. Glera) and rootstock (cv. Kober 5BB) (by DNA sequencing technologies and intelligent computing) was preliminary analyzed just before grafting, in order to detect differences on the fungal and bacterial community prior treatment. For the second aim, three methods of grafting, (i) Omega graft, (ii) Whip and Tongue graft and (iii) Full cleft graft, were compared by observing xylogenesis and vascular tissue formation at the grafting point, in order to detect the factors potentially influencing the vine physiology. Early results showed a distinct profile in fungi and bacteria for both rootstock and scion. In rootstock, a higher abundance of GTDs agents was detected, especially in the bark. The anatomical observation on grapevine cuttings, immediately after callusing, revealed differences between all the three grafting methods: the full cleft graft showed the most complete and homogenous callus, which is the ideal condition to avoid the presence of necrosis, potentially colonized by the GTDs pathogens.
Monitoring and preventing grapevine trunk diseases in the nursery: comparative assays to control the related pathogens in propagation material
Di Marco S;
2019
Abstract
The control of grapevine trunk diseases (GTDs) represents a big challenge for grapevine nurseries due to the high infection risks on the propagating material. Plant material disinfection before storage and the grafting practice are both potential factors able to affect the future GTDs development in young vines in field. In this framework, this research is aimed to evaluate the microbiome (especially taking into account GTDs pathogens) in the propagation material undergone through different disinfection treatments, and to compare the impact of three grafting methods on the formation of an active and functional vascular system. To assess the first aim, the microbiome of the scion (cv. Glera) and rootstock (cv. Kober 5BB) (by DNA sequencing technologies and intelligent computing) was preliminary analyzed just before grafting, in order to detect differences on the fungal and bacterial community prior treatment. For the second aim, three methods of grafting, (i) Omega graft, (ii) Whip and Tongue graft and (iii) Full cleft graft, were compared by observing xylogenesis and vascular tissue formation at the grafting point, in order to detect the factors potentially influencing the vine physiology. Early results showed a distinct profile in fungi and bacteria for both rootstock and scion. In rootstock, a higher abundance of GTDs agents was detected, especially in the bark. The anatomical observation on grapevine cuttings, immediately after callusing, revealed differences between all the three grafting methods: the full cleft graft showed the most complete and homogenous callus, which is the ideal condition to avoid the presence of necrosis, potentially colonized by the GTDs pathogens.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
