In the table grape (Vitis vinifera) market, seedlessness is greatly prized by consumers of fresh fruits, thus causing an ever increasing demand of seedless varieties in recent years. Two kinds of seedlessness are known in grapevine: the partenocarpy, for which ovary is stimulated to develop without ovule fertilization (small size berries are formed and they are usually used for raisin production), and the stenospermocarpy, in which fertilization normally occurs, but embryo prematurely aborts and seed development stops (normal size berries, compatible with commercial requirement, are able to develop). Seedlessness is the result of complex developmental processes modified by genetic, physiological and environmental factors. Three independent and complementary recessive genes regulated by a dominant locus, named SDI (Seed Development Inhibitor), seem to be involved in the genetic control of the stenospermocarpic seed abortion in commercial grapevine cultivars. A MADS-box locus (VvAGL11) has been proposed as the major functional candidate for grape seedlessness; it also includes a (GAGA)n motif, proposed as the most useful molecular marker in breeding program. The use of this intragenic microsatellite for Marker Assisted Selection (MAS) is an extremely effective method to accelerate and simplify the selection processes of interesting genotypes, especially with regard to traits with tardive phenotypic manifestation as seedlessness. In this research two different strategies for SSR analysis have been compared: I) capillary electrophoresis e II) High Resolution Melting (HRM). Parameters such as simplicity, accurateness, sensitiveness, specificity and costs have been evaluated. Moreover, a CAPS marker, known to co-localized with VvAGL11 and to be fully linked to the presence of seedless haplotype, has been applied for genotyping the analysed cultivars.
Molecular strategies for fast and cheap screening of seedlessed grapes.
SABETTA W;
2019
Abstract
In the table grape (Vitis vinifera) market, seedlessness is greatly prized by consumers of fresh fruits, thus causing an ever increasing demand of seedless varieties in recent years. Two kinds of seedlessness are known in grapevine: the partenocarpy, for which ovary is stimulated to develop without ovule fertilization (small size berries are formed and they are usually used for raisin production), and the stenospermocarpy, in which fertilization normally occurs, but embryo prematurely aborts and seed development stops (normal size berries, compatible with commercial requirement, are able to develop). Seedlessness is the result of complex developmental processes modified by genetic, physiological and environmental factors. Three independent and complementary recessive genes regulated by a dominant locus, named SDI (Seed Development Inhibitor), seem to be involved in the genetic control of the stenospermocarpic seed abortion in commercial grapevine cultivars. A MADS-box locus (VvAGL11) has been proposed as the major functional candidate for grape seedlessness; it also includes a (GAGA)n motif, proposed as the most useful molecular marker in breeding program. The use of this intragenic microsatellite for Marker Assisted Selection (MAS) is an extremely effective method to accelerate and simplify the selection processes of interesting genotypes, especially with regard to traits with tardive phenotypic manifestation as seedlessness. In this research two different strategies for SSR analysis have been compared: I) capillary electrophoresis e II) High Resolution Melting (HRM). Parameters such as simplicity, accurateness, sensitiveness, specificity and costs have been evaluated. Moreover, a CAPS marker, known to co-localized with VvAGL11 and to be fully linked to the presence of seedless haplotype, has been applied for genotyping the analysed cultivars.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
