Olfactory Ensheathing Cells (OECs), glial cells of the olfactory system, are able to secrete several neurotrophic growth factors, promoting axonal growth and supporting remyelination of damaged axons. In the olfactory system, during the development, they drive the non-myelinated axons of the Olfactory Receptor Neurons towards the Olfactory bulbs. OEC transplantation has emerged as a possible experimental therapy to induce repair of nervous system injury, even if the functional recovery is still limited. Several studies suggest that into all the olfactory system play an important role Ghrelin (Ghre), an orexigenic peptide that acts not only in the olfactory cerebral areas, but also on the most peripheral part of the olfactory system. It is a polypeptide and a gut hormone produced from the stomach, it is able to reach the Central Nervous System by regulating metabolism and by performing neuroprotection. Our previous investigations have shown that OB mitral cells express Ghre, thus being able to play an important role in regulating food behavior in response to odors. The aim of this study was to investigate on the secretion and the presence of Ghre peptide and its receptors in OECs. Primary OECs were obtained from 2-d old mice pup (P2) olfactory bulbs, the cells were grown in Dulbecco's Modified Eagle's medium supplemented with 10% Foetal Bovine Serum. Immunocytochemical and Western blot analysis were used. Our results, for the first time, showed that OECs were able to secrete the Ghre peptide and that OECs express GHS-R 1a receptors. Moreover, being known that Ghre has a neurotrophic action, both in promoting axonal growth and synaptogenesis probably through the secreting factors, it could be hypothesized that even the OECs through the Ghre, can improve the growth of neurites and promote alignment. Therefore, it can be hypothesized that they can interact with reinforcement function, in the peripheral olfactory circuit, providing a neurotrophic support to the synaptic interaction between the ORNs and the mitral cells.
Do Olfactory Ensheathing Cells express Ghrelin? An in vitro investigation.
2019
Abstract
Olfactory Ensheathing Cells (OECs), glial cells of the olfactory system, are able to secrete several neurotrophic growth factors, promoting axonal growth and supporting remyelination of damaged axons. In the olfactory system, during the development, they drive the non-myelinated axons of the Olfactory Receptor Neurons towards the Olfactory bulbs. OEC transplantation has emerged as a possible experimental therapy to induce repair of nervous system injury, even if the functional recovery is still limited. Several studies suggest that into all the olfactory system play an important role Ghrelin (Ghre), an orexigenic peptide that acts not only in the olfactory cerebral areas, but also on the most peripheral part of the olfactory system. It is a polypeptide and a gut hormone produced from the stomach, it is able to reach the Central Nervous System by regulating metabolism and by performing neuroprotection. Our previous investigations have shown that OB mitral cells express Ghre, thus being able to play an important role in regulating food behavior in response to odors. The aim of this study was to investigate on the secretion and the presence of Ghre peptide and its receptors in OECs. Primary OECs were obtained from 2-d old mice pup (P2) olfactory bulbs, the cells were grown in Dulbecco's Modified Eagle's medium supplemented with 10% Foetal Bovine Serum. Immunocytochemical and Western blot analysis were used. Our results, for the first time, showed that OECs were able to secrete the Ghre peptide and that OECs express GHS-R 1a receptors. Moreover, being known that Ghre has a neurotrophic action, both in promoting axonal growth and synaptogenesis probably through the secreting factors, it could be hypothesized that even the OECs through the Ghre, can improve the growth of neurites and promote alignment. Therefore, it can be hypothesized that they can interact with reinforcement function, in the peripheral olfactory circuit, providing a neurotrophic support to the synaptic interaction between the ORNs and the mitral cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
