In the last few years, Ghrelin (Ghre), a gut-brain hormone, has attracted particular interest for its neuroprotective, antioxidant, anti-inflammatory, and anti-apoptotic properties. This orexigenic peptide plays an important role within the olfactory system and in specific neuronal populations of the hypothalamus, amygdala and hippocampus. Our previous investigation showed that a conditioned medium (CM) from glial cells, such as Schwann cells (SCs) and Olfactory Ensheathing Cells (OECs) promotes a neural differentiation of Adipose-derived mesenchymal Stem Cells (ASCs). The aim of this study was to evaluate the effect of Ghre on ASC cultures. ASCs were isolated after collagenase digestion and centrifugation of the raw lipoaspirate. Different culture conditions were tested: ASCs cultured either in the basal medium, or in OEC-CM or SC-CM were considered as controls. In three other corresponding ASC culture samples, Ghre (2 ?M) was added. After 1, 3 and 6 days of growth, cells were tested by immunocytochemistry to detect the expression of some neural markers, such as Protein Gene Product (PGP) 9.5, Microtubule Associated Protein (MAP) 2, Glial Fibrillary Acidic Protein (GFAP), and Neuron Specific Enolase (NSE). The expression of Ghre and its receptor was also evaluated. Results confirmed that both OEC-CM and SC-CM increased the expression of neural markers in ASCs. These increases were more evident when Ghre was added, although ASCs treated with Ghre alone exhibited weak modifications. Improvements were clearly visible already at day 1 and were more evident at day 6. At least in part, the observed effects are likely due to interactions between Ghre and its receptor, whose expression was also increased. In conclusion, although Ghre alone was not capable of inducing pronounced effects, its addition facilitated neural ASC differentiation. This study provides information on the synergic action of Ghre and glia CM on modulation of ASC growth and differentiation.
Synergic effect of glia conditioned media and Ghrelin on marker expression of Adipose-derived mesenchymal Stem Cells.
Rosalia Pellitteri
2019
Abstract
In the last few years, Ghrelin (Ghre), a gut-brain hormone, has attracted particular interest for its neuroprotective, antioxidant, anti-inflammatory, and anti-apoptotic properties. This orexigenic peptide plays an important role within the olfactory system and in specific neuronal populations of the hypothalamus, amygdala and hippocampus. Our previous investigation showed that a conditioned medium (CM) from glial cells, such as Schwann cells (SCs) and Olfactory Ensheathing Cells (OECs) promotes a neural differentiation of Adipose-derived mesenchymal Stem Cells (ASCs). The aim of this study was to evaluate the effect of Ghre on ASC cultures. ASCs were isolated after collagenase digestion and centrifugation of the raw lipoaspirate. Different culture conditions were tested: ASCs cultured either in the basal medium, or in OEC-CM or SC-CM were considered as controls. In three other corresponding ASC culture samples, Ghre (2 ?M) was added. After 1, 3 and 6 days of growth, cells were tested by immunocytochemistry to detect the expression of some neural markers, such as Protein Gene Product (PGP) 9.5, Microtubule Associated Protein (MAP) 2, Glial Fibrillary Acidic Protein (GFAP), and Neuron Specific Enolase (NSE). The expression of Ghre and its receptor was also evaluated. Results confirmed that both OEC-CM and SC-CM increased the expression of neural markers in ASCs. These increases were more evident when Ghre was added, although ASCs treated with Ghre alone exhibited weak modifications. Improvements were clearly visible already at day 1 and were more evident at day 6. At least in part, the observed effects are likely due to interactions between Ghre and its receptor, whose expression was also increased. In conclusion, although Ghre alone was not capable of inducing pronounced effects, its addition facilitated neural ASC differentiation. This study provides information on the synergic action of Ghre and glia CM on modulation of ASC growth and differentiation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
