Background: In the frame of a research line dedicated to better clarify the role of exopolysaccharides (EPS) in bacterial virulence, EPS produced by species of the Burkholderia cepacia complex (Bcc), namely Burkholderia multivorans, Burkholderia cenocepacia, and a Bcc member of undetd. genomovar, all isolated at the Cystic Fibrosis Regional Center of Florence (Italy), were investigated for they structural properties. Methods: Three strains of B. multivorans, three of B. cenocepacia and one of a Bcc member of undetd. genomovar were isolated from CF patients. The ref. strains C1576 and J2315, for genomovar II and III, resp., were included in the study. The bacteria were grown on solid media, the exopolysaccharides produced were purified, and their structures were detd. In addn., sugar anal. of sputum samples was accomplished to search for EPS produced in vivo. Results: Six strains out of seven produced the exopolysaccharide cepacian, while one strain of B. multivorans produced a completely different polymer, previously known in the literature as PS1. Two strains synthesized very small amts. of EPS. No definitive evidence for the presence of cepacian in sputum samples was found. Conclusions: Most strains examd. produced abundant amts. of polysaccharides. Cepacian was the most common EPS isolated and its prodn. was not assocd. to a particular genomovar.
Exopolysaccharides produced by clinical strains belonging to the Burkholderia cepacia complex
G Impallomeni;
2007
Abstract
Background: In the frame of a research line dedicated to better clarify the role of exopolysaccharides (EPS) in bacterial virulence, EPS produced by species of the Burkholderia cepacia complex (Bcc), namely Burkholderia multivorans, Burkholderia cenocepacia, and a Bcc member of undetd. genomovar, all isolated at the Cystic Fibrosis Regional Center of Florence (Italy), were investigated for they structural properties. Methods: Three strains of B. multivorans, three of B. cenocepacia and one of a Bcc member of undetd. genomovar were isolated from CF patients. The ref. strains C1576 and J2315, for genomovar II and III, resp., were included in the study. The bacteria were grown on solid media, the exopolysaccharides produced were purified, and their structures were detd. In addn., sugar anal. of sputum samples was accomplished to search for EPS produced in vivo. Results: Six strains out of seven produced the exopolysaccharide cepacian, while one strain of B. multivorans produced a completely different polymer, previously known in the literature as PS1. Two strains synthesized very small amts. of EPS. No definitive evidence for the presence of cepacian in sputum samples was found. Conclusions: Most strains examd. produced abundant amts. of polysaccharides. Cepacian was the most common EPS isolated and its prodn. was not assocd. to a particular genomovar.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
