The development of novel nanomaterials for new and smart formulations to overcome the limitations of organic chromophores, and significantly accelerate their clinical translation, have attracted enormous interest in the last two decades. Efficient assembly in host-guest interactions is crucial to supramolecular nanotechnology. Cyclodextrins (CDs) improve the biocompatibility of nanodelivery systems, and hence, supramolecular approaches utilizing CDs can improve and expand the design and applications of functional delivery systems [1]. Aim of this work was to evaluate the feasibility to obtain a biocompatible nanogel for photodynamic antimicrobial therapy (PAT) able to promote internalization in cell/bacterial with biocompatible nanoformulations based on CAPTISOL® (sulphobutylether-beta-cyclodextrin, SBE-beta-CD). PAT mechanism involves a photosensitizer (PS), bound to the colonized surface, which is promoted to its excited singlet state (1PS) by shining light on its absorption bands. This species evolves to the triplet state (3PS) that can decay by transferring energy to the surrounding oxygen and molecular components. [2,3]. Herein, we aimed to prepare novel supramolecular nanogel by exploiting a supramolecular interaction between CAPTISOL® with the tetracationic water soluble meso-tetrakis(N-methylpyridinium- 4-yl)porphine (TMpyP) as photosensitizer and by using a surfactant which acts as cross-linking agent. First of all, the ability of CAPTISOL® to form complexes with PS as building block of nanoassemblies were studied with complementary techniques such as UV-Vis, steady-state fluorescence spectroscopy, anisotropy, resonance light scattering (RLS). In aqueous solution, a phase solubility study was carried out to evaluate the enhancement of the solubility of TMpyP and, therefore, the occurred complexation with the macrocycle. The stoichiometry and the stability constant of the CAPTISOL®/TMpyP complex were calculated with the phase solubility method and through the Job's plot. The spectral changes induced by complexation were investigated by complementary spectroscopy techniques. Nanoassemblies were characterized, in order to elucidate size, drug loading and sites of PS entrapment in CDs. Currently, biological evaluation of this supramolecular system on different cancer cell lines is in progress.
Novel Nanogel based on CAPTISOL® and a Cationic Poprhyrin with the Potential for PAT
Roberto Zagami;Antonino Mazzaglia
2018
Abstract
The development of novel nanomaterials for new and smart formulations to overcome the limitations of organic chromophores, and significantly accelerate their clinical translation, have attracted enormous interest in the last two decades. Efficient assembly in host-guest interactions is crucial to supramolecular nanotechnology. Cyclodextrins (CDs) improve the biocompatibility of nanodelivery systems, and hence, supramolecular approaches utilizing CDs can improve and expand the design and applications of functional delivery systems [1]. Aim of this work was to evaluate the feasibility to obtain a biocompatible nanogel for photodynamic antimicrobial therapy (PAT) able to promote internalization in cell/bacterial with biocompatible nanoformulations based on CAPTISOL® (sulphobutylether-beta-cyclodextrin, SBE-beta-CD). PAT mechanism involves a photosensitizer (PS), bound to the colonized surface, which is promoted to its excited singlet state (1PS) by shining light on its absorption bands. This species evolves to the triplet state (3PS) that can decay by transferring energy to the surrounding oxygen and molecular components. [2,3]. Herein, we aimed to prepare novel supramolecular nanogel by exploiting a supramolecular interaction between CAPTISOL® with the tetracationic water soluble meso-tetrakis(N-methylpyridinium- 4-yl)porphine (TMpyP) as photosensitizer and by using a surfactant which acts as cross-linking agent. First of all, the ability of CAPTISOL® to form complexes with PS as building block of nanoassemblies were studied with complementary techniques such as UV-Vis, steady-state fluorescence spectroscopy, anisotropy, resonance light scattering (RLS). In aqueous solution, a phase solubility study was carried out to evaluate the enhancement of the solubility of TMpyP and, therefore, the occurred complexation with the macrocycle. The stoichiometry and the stability constant of the CAPTISOL®/TMpyP complex were calculated with the phase solubility method and through the Job's plot. The spectral changes induced by complexation were investigated by complementary spectroscopy techniques. Nanoassemblies were characterized, in order to elucidate size, drug loading and sites of PS entrapment in CDs. Currently, biological evaluation of this supramolecular system on different cancer cell lines is in progress.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
