Grapevine trunk disease management has several critical aspects, and one of the main ones is the lack of suitable products with low toxicity but good efficacy. This indicated a need to investigate the applicability of micro-structured inorganic crystals based on a specific apatitic phase of calcium phosphate (carbonate hydroxyapatite, CHA) in plant protection, already in use in the medical field. To this aim, several formulations were prepared, functionalizing CHA with two different copper salts (tribasic sulphate and sulphate pentahydrate) and uploading afterwards zinc sulphate and a commercial essential oil product. The formulations were applied successfully in downy mildew control trials on grapevine to investigate their drug-delivery proprieties in enhancing the efficacy of bioactive substances. The most stable formulations were obtained with copper tribasic sulphate and zinc sulphate. The essential oil seems to only bind with CHA by physical interactions. The formulations were also tested in vitro to evaluate the possible application in GTD pathogens control in the nursery. Growth inhibition tests were carried out on Phaeomoniella chlamydospora, Phaeoacremonium minimum and Neofusicoccum parvum, and also on Botrytis cinerea, given the great relevance of this pathogen in the nursery. In vitro tests showed CHA stimulated the growth of all the fungi assessed, and the most efficient formulation was based on CHA and copper sulphate, followed by the CHA and copper tribasic sulphate based formulation. The formulations were applied in the nursery during the hydration of the propagating material, a proportion of which was afterwards artifciially inoculated with P. chlamydospora. None of the treatments showed phytotoxic effect on plant growth and yield. Leaves where sampled from the plants for transcriptomic analysis (RT-PCR) in order to evaluate their effect on the plant defense response. Furthermore data were obtained on reisolating P. chlamydospora from the inoculated propagation material. The pathogen reisolation confirmed the in vitro test results, revealing the non-toxic effct of the pure CHA and positive pathogen control enhanced by the formulation CHA-copper sulphate pentahydrate.
Developing a delivery system for the control of plant diseases: from leaf pathogen control to grapevine trunk diseases control in the nursery.
F OSTI;S DI MARCO
2017
Abstract
Grapevine trunk disease management has several critical aspects, and one of the main ones is the lack of suitable products with low toxicity but good efficacy. This indicated a need to investigate the applicability of micro-structured inorganic crystals based on a specific apatitic phase of calcium phosphate (carbonate hydroxyapatite, CHA) in plant protection, already in use in the medical field. To this aim, several formulations were prepared, functionalizing CHA with two different copper salts (tribasic sulphate and sulphate pentahydrate) and uploading afterwards zinc sulphate and a commercial essential oil product. The formulations were applied successfully in downy mildew control trials on grapevine to investigate their drug-delivery proprieties in enhancing the efficacy of bioactive substances. The most stable formulations were obtained with copper tribasic sulphate and zinc sulphate. The essential oil seems to only bind with CHA by physical interactions. The formulations were also tested in vitro to evaluate the possible application in GTD pathogens control in the nursery. Growth inhibition tests were carried out on Phaeomoniella chlamydospora, Phaeoacremonium minimum and Neofusicoccum parvum, and also on Botrytis cinerea, given the great relevance of this pathogen in the nursery. In vitro tests showed CHA stimulated the growth of all the fungi assessed, and the most efficient formulation was based on CHA and copper sulphate, followed by the CHA and copper tribasic sulphate based formulation. The formulations were applied in the nursery during the hydration of the propagating material, a proportion of which was afterwards artifciially inoculated with P. chlamydospora. None of the treatments showed phytotoxic effect on plant growth and yield. Leaves where sampled from the plants for transcriptomic analysis (RT-PCR) in order to evaluate their effect on the plant defense response. Furthermore data were obtained on reisolating P. chlamydospora from the inoculated propagation material. The pathogen reisolation confirmed the in vitro test results, revealing the non-toxic effct of the pure CHA and positive pathogen control enhanced by the formulation CHA-copper sulphate pentahydrate.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
