Plant nanobionics is a promising technology based on the combination of plant cell with nanomaterials. Nanomaterials, in particular carbon based nanomaterials integrated within plant cells were proposed as active system able to influence plant growth, increase the water retaining capacity and stimulate gene and protein expression. Single wall carbon nanotubes (SWCNT) were successfully used to deliver into plants small molecules, fertilizers or pesticides. Despite the great potential deriving from the use of nanotubes in the cultivation of photosynthetic microorganisms, many questions remain open and in need of answers1. In particular, the relationship between the chemical-physical properties of nanotubes and their biological behaviour is still unclear. Finally, it would be appropriate to develop convenient and fast methodology able to monitor, possibly in real-time, cellular absorption and biological effect of nanotubes in cell. Herein, we provide an experimental approach suitable to support nanomaterial-based applications promoting algal and plant biotechnology, and risk assessment studies on CNTs phytotoxicity. The strategy we considered here employs the robust and cost-affordable conventional Raman to track the cellular internalization of SWCNTs. This approach allows advance in the understanding of the relationships among physicochemical features of CNTs and their cellular internalization capacity and ability to interact with the cell components in vivo. SWCNTs may be proposed as effective in vivo spectroscopic probes, able to vanish the fluorescence background of the photosynthetic cell usually affecting visible spectroscopic analyses. [1]M. D. Lambreva, T. Lavecchia, E. Tyystjärvi, T. K. Antal, S. Orlanducci, A. Margonelli, G. Rea, Photosynth. Res. 2015, 125, 451. Acknowledgements: This work was supported by the program for Bilateral Scientific and Technological Cooperation CNR-RFBR within the Joint Research Project Lambreva/Rubin, 2015-2017. TKA acknowledges the RSF Grant number: 14-50-00029.
SWCNT as Raman probe in photosynthetic cell
Andrea Margonelli;Giuseppina Rea;
2018
Abstract
Plant nanobionics is a promising technology based on the combination of plant cell with nanomaterials. Nanomaterials, in particular carbon based nanomaterials integrated within plant cells were proposed as active system able to influence plant growth, increase the water retaining capacity and stimulate gene and protein expression. Single wall carbon nanotubes (SWCNT) were successfully used to deliver into plants small molecules, fertilizers or pesticides. Despite the great potential deriving from the use of nanotubes in the cultivation of photosynthetic microorganisms, many questions remain open and in need of answers1. In particular, the relationship between the chemical-physical properties of nanotubes and their biological behaviour is still unclear. Finally, it would be appropriate to develop convenient and fast methodology able to monitor, possibly in real-time, cellular absorption and biological effect of nanotubes in cell. Herein, we provide an experimental approach suitable to support nanomaterial-based applications promoting algal and plant biotechnology, and risk assessment studies on CNTs phytotoxicity. The strategy we considered here employs the robust and cost-affordable conventional Raman to track the cellular internalization of SWCNTs. This approach allows advance in the understanding of the relationships among physicochemical features of CNTs and their cellular internalization capacity and ability to interact with the cell components in vivo. SWCNTs may be proposed as effective in vivo spectroscopic probes, able to vanish the fluorescence background of the photosynthetic cell usually affecting visible spectroscopic analyses. [1]M. D. Lambreva, T. Lavecchia, E. Tyystjärvi, T. K. Antal, S. Orlanducci, A. Margonelli, G. Rea, Photosynth. Res. 2015, 125, 451. Acknowledgements: This work was supported by the program for Bilateral Scientific and Technological Cooperation CNR-RFBR within the Joint Research Project Lambreva/Rubin, 2015-2017. TKA acknowledges the RSF Grant number: 14-50-00029.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
