Antioxidant therapy is a modern method, effective in preventing the abnormal production of aggressive reactive oxygen species and limiting their harmful effects on the human body. In this regard, the treatment of a number of diseases is based on "traditional" drugs with targeted functionality and various antioxidants. Potential application of mixtures of phenolic antioxidants (bio-antioxidants) can be assessed in phytochemical therapies or in specific diets recommended for metabolic disorders. This study aims to create new effective bio-antioxidant compositions based on physiologically important compounds like ascorbic acid (AA) and natural or recently synthesized natural-like phenols as hydroxychavicol (HCh) and its dimer (DHCh) respectively, combined with 5-fluorouracyl (5-FU) - a common chemotherapy drug. Our previous investigations showed that 5-FU was not soluble in lipid substrate and the experiments of bulk lipid autoxidation in presence of 5-FU were made in presence of DMSO. Unfortunately, DMSO reduced the antioxidant potential of phenolic compounds. For that reason in this study we solved 5-FU in acetone solution of AA. Last was used as an amphiphilic compound because of its high solubility in water and in a lipid substrate at low concentrations (<0.3mM). The process was performed in a kinetic regime (in Petri dishes, with kinetically pure triacylglycerols of sunflower oil as lipid substrate, in the dark, at room temperature) in the presence of binary and/or ternary mixtures containing the substances mentioned above in different combinations. The presence of 5-FU did not affect the inhibition degree and the antioxidant reactivity of hydroxychavicol (HCh) and its dimer (DHCh) during the initial stage of the oxidation process.
NEW ANTIOXIDANT COMPOSITIONS OF 5-FLUOROURACYL AND ASCORBIC ACID IN PRESENCE OF HYDROXYCHAVICOL OR HYDROXYCHAVICOL DIMER
Davide Fabbri;Maria Antonietta Dettori;
2018
Abstract
Antioxidant therapy is a modern method, effective in preventing the abnormal production of aggressive reactive oxygen species and limiting their harmful effects on the human body. In this regard, the treatment of a number of diseases is based on "traditional" drugs with targeted functionality and various antioxidants. Potential application of mixtures of phenolic antioxidants (bio-antioxidants) can be assessed in phytochemical therapies or in specific diets recommended for metabolic disorders. This study aims to create new effective bio-antioxidant compositions based on physiologically important compounds like ascorbic acid (AA) and natural or recently synthesized natural-like phenols as hydroxychavicol (HCh) and its dimer (DHCh) respectively, combined with 5-fluorouracyl (5-FU) - a common chemotherapy drug. Our previous investigations showed that 5-FU was not soluble in lipid substrate and the experiments of bulk lipid autoxidation in presence of 5-FU were made in presence of DMSO. Unfortunately, DMSO reduced the antioxidant potential of phenolic compounds. For that reason in this study we solved 5-FU in acetone solution of AA. Last was used as an amphiphilic compound because of its high solubility in water and in a lipid substrate at low concentrations (<0.3mM). The process was performed in a kinetic regime (in Petri dishes, with kinetically pure triacylglycerols of sunflower oil as lipid substrate, in the dark, at room temperature) in the presence of binary and/or ternary mixtures containing the substances mentioned above in different combinations. The presence of 5-FU did not affect the inhibition degree and the antioxidant reactivity of hydroxychavicol (HCh) and its dimer (DHCh) during the initial stage of the oxidation process.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
