Temporary Immersion System for Micropropagation of Tree Species: a Bibliographic and Systematic Review

This paper was characterized by a bibliometric and systematic review on the database ISI Web of Science, aiming toprovide an update of the main points addressed regarding the Temporary Immersion Systems (TIS) for micropropagation oftree species. It was pointed out that the frequency and time of immersion were one of the main parameters studied in thepapers and 35% these papers worked with eucalyptus species. The main problem reported in the papers was the hyperhydricity,but it was overcome via procedures such as: a) air injection into the system, b) increasing the immersion intervals anddecreasing the immersion time and c) decreasing concentration of cytokinin. Most papers reported that TIS produced plantsthat were more successful in surviving the ex-vitro acclimation stage than those produced on semi-solid media or continuousimmersion systems. Few studies compared different types of temporary immersion bioreactors on micropropagation of plantsand within the established criteria, papers with TIS tree species represented only 15% of the total. This system has presentedpromising results for most of the tree species, and although some gaps have been identified and few are the works with treespecies, this process has been taking an increasingly larger space in the propagation of plants.