We report here a new procedure for THz timedomain spectroscopic analysis of mammalian cell samples exposed to electroporating pulsed electric fields. Suspensions of human monocytes, Mono-Mac 6 (MM-6), were exposed to 8, 100 ?s, 400 V (100 kV/m between the cuvette electrodes) pulses at 5 kHz, and then their spectral response between 0.2 and 1.5 THz was recorded with a commercial THz time-domain spectrometer. Pulse-exposed cells absorb more THz radiation than sham-exposed cells, suggesting that THz spectroscopy can be used as a powerful, labelfree method for characterization of electroporation.
Electroporation detection with THz timedomain spectroscopy
Stefania Romeo;Olga Zeni
2018
Abstract
We report here a new procedure for THz timedomain spectroscopic analysis of mammalian cell samples exposed to electroporating pulsed electric fields. Suspensions of human monocytes, Mono-Mac 6 (MM-6), were exposed to 8, 100 ?s, 400 V (100 kV/m between the cuvette electrodes) pulses at 5 kHz, and then their spectral response between 0.2 and 1.5 THz was recorded with a commercial THz time-domain spectrometer. Pulse-exposed cells absorb more THz radiation than sham-exposed cells, suggesting that THz spectroscopy can be used as a powerful, labelfree method for characterization of electroporation.File in questo prodotto:
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