The effect of an abomecttn formulation (l/ertiruec@ EC) was tested against the cyst nemalodes Globodera pallida Heterodera carotae and Hetercdera schachtii in an in vito hatching test. Abamectin (a mixture of two macrocyclic lactones Bla and Blb) is produced by the actinomycete Streptomyees avermitilis. Cysts of the nematodes were extracted from sarnples of infested soils by the Fenwick can and then subjected ta dffirent concentratiow of an oqueous solution of the abomeetinfortnulation (A, 1.125, 2.25, 4.5, 9.0, 18.0 and 36 g/mL) and exposure ttmesfrom 24 to 384 hours. For each nematode specie batches of 5A cysts were set up and arranged in a growth cabinet (20 oC) according to o randomized block design. Un*eated cysts were used as cantrol. There were three repltcations for each treatment. As natural and artificial hotching agents for H. carotae, H. schachtii and G. pallida were used caftot raot leachate, 0.3 mM zinc chloride and 0"6 mM sodium metavanadate aqueoas solutions, respectively. Every week ewerged juveniles were counted. At the end of the hatching test cysts were crushed and unhatched eggs and juveniles counted. The total number of jweniles emerged in the hatchtng test were expressed as the percentage of the total egg corrtefi of the cysts (hatched + unhatched). From percentage hatch was caleulated the mortality of each treatment considering the natural death in the cantrol. Data af percentage mortaltty were subjected to probit analysis to calculate values af lethal doses (LD) required for 50 % egg mortality. At 24 hours exposure, values of LD50 for H. carotae, G. pallida and H. schachtii were 9.9, i,3,2 and 796 microg/ml, respectively. For the same nematode species at 384 hours exposure LD50 decreased at 3.6, 2.9 and 17.6 microg/ml.
In vitro effect of abamectin from Streptomyces avermitilis on the survival of the cyst nematodes Globodera pallida, Heterodera carotae and Heterodera schachtii
SASANELLI N;
2020
Abstract
The effect of an abomecttn formulation (l/ertiruec@ EC) was tested against the cyst nemalodes Globodera pallida Heterodera carotae and Hetercdera schachtii in an in vito hatching test. Abamectin (a mixture of two macrocyclic lactones Bla and Blb) is produced by the actinomycete Streptomyees avermitilis. Cysts of the nematodes were extracted from sarnples of infested soils by the Fenwick can and then subjected ta dffirent concentratiow of an oqueous solution of the abomeetinfortnulation (A, 1.125, 2.25, 4.5, 9.0, 18.0 and 36 g/mL) and exposure ttmesfrom 24 to 384 hours. For each nematode specie batches of 5A cysts were set up and arranged in a growth cabinet (20 oC) according to o randomized block design. Un*eated cysts were used as cantrol. There were three repltcations for each treatment. As natural and artificial hotching agents for H. carotae, H. schachtii and G. pallida were used caftot raot leachate, 0.3 mM zinc chloride and 0"6 mM sodium metavanadate aqueoas solutions, respectively. Every week ewerged juveniles were counted. At the end of the hatching test cysts were crushed and unhatched eggs and juveniles counted. The total number of jweniles emerged in the hatchtng test were expressed as the percentage of the total egg corrtefi of the cysts (hatched + unhatched). From percentage hatch was caleulated the mortality of each treatment considering the natural death in the cantrol. Data af percentage mortaltty were subjected to probit analysis to calculate values af lethal doses (LD) required for 50 % egg mortality. At 24 hours exposure, values of LD50 for H. carotae, G. pallida and H. schachtii were 9.9, i,3,2 and 796 microg/ml, respectively. For the same nematode species at 384 hours exposure LD50 decreased at 3.6, 2.9 and 17.6 microg/ml.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
