5',8-cyclopurines (cPu) are tandem-type lesions observed among the DNA modifications. These lesions are generated by the reaction of HOo radicals with the genetic material via C5' radical chemistry and can be present in two diasteroisomeric forms 5'R and 5'S for each 5',8-cyclo-2'-deoxyadenosine (cdA) and 5',8-cyclo-2'-deoxyguanosine (cdG). This chapter describes the analytical protocols for the quantitative determination and detection limits of the levels of lesions in various types of cells and animal model systems, and relationships between the levels of lesions and human health, disease, and aging. The cPu lesions identified in mammalian cellular DNA in vivo, can be repaired only by nucleotide excision repair (NER) enzyme with low efficiencies and their accumulation in the genome results in severe adverse effects on cellular functions. The use of these lesions as candidate biomarkers of DNA damage is increasingly appreciated because the cPu DNA lesions do not suffer from stability issues and artifacts of other oxidatively generated DNA lesions.
Oxidation of the C5' Position in DNA and the Role of Purine 5',8-Cyclo-2'-deoxynucleoside Lesions
Chatgilialoglu C;Masi A
2021
Abstract
5',8-cyclopurines (cPu) are tandem-type lesions observed among the DNA modifications. These lesions are generated by the reaction of HOo radicals with the genetic material via C5' radical chemistry and can be present in two diasteroisomeric forms 5'R and 5'S for each 5',8-cyclo-2'-deoxyadenosine (cdA) and 5',8-cyclo-2'-deoxyguanosine (cdG). This chapter describes the analytical protocols for the quantitative determination and detection limits of the levels of lesions in various types of cells and animal model systems, and relationships between the levels of lesions and human health, disease, and aging. The cPu lesions identified in mammalian cellular DNA in vivo, can be repaired only by nucleotide excision repair (NER) enzyme with low efficiencies and their accumulation in the genome results in severe adverse effects on cellular functions. The use of these lesions as candidate biomarkers of DNA damage is increasingly appreciated because the cPu DNA lesions do not suffer from stability issues and artifacts of other oxidatively generated DNA lesions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.