Two in vitro experiments and two open field trials were carried out to investigate the nematicidal effect of Ruta graveolens on the root-knot nematode Meloidogyne incognita. In the first in vitro experiment the nematode egg masses were immersed for 3 weeks in aqueous leaf extract of rue plants and then subjected to an hatching test in distilled water for five more weeks. In the other in vitro experiment M. incognita egg masses were exposed to the rue extract for 4, 8 and 16 hours and then incubated in distilled water for 8 weeks. Distilled water and a 5 mkg/mL an aqueous solution of Fenamiphos or only distilled water were used as controls in the first and second in vitro experiment, respectively. Both hatching tests were carried out in a growth cabinet at 25+2 0C, providing four replicates for each treatment. In the first experiment the hatching from the egg masses treated with rue extracts and Fenamiphos solution resulted significantly lower than that in the untreated control.
ACTIVITY OF RUTA GRAVEOLENS L. AGAINST ROOT-KNOT NEMATODES (MELOIDOGYNE SPP.)
D'ADDABBO T;
2020
Abstract
Two in vitro experiments and two open field trials were carried out to investigate the nematicidal effect of Ruta graveolens on the root-knot nematode Meloidogyne incognita. In the first in vitro experiment the nematode egg masses were immersed for 3 weeks in aqueous leaf extract of rue plants and then subjected to an hatching test in distilled water for five more weeks. In the other in vitro experiment M. incognita egg masses were exposed to the rue extract for 4, 8 and 16 hours and then incubated in distilled water for 8 weeks. Distilled water and a 5 mkg/mL an aqueous solution of Fenamiphos or only distilled water were used as controls in the first and second in vitro experiment, respectively. Both hatching tests were carried out in a growth cabinet at 25+2 0C, providing four replicates for each treatment. In the first experiment the hatching from the egg masses treated with rue extracts and Fenamiphos solution resulted significantly lower than that in the untreated control.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
