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The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Thery Clotilde;Witwer Kenneth W;Aikawa Elena;Jose Alcaraz Maria;Anderson Johnathon D;Andriantsitohaina Ramaroson;Antoniou Anna;Arab Tanina;Archer Fabienne;AtkinSmith Georgia K;Ayre D Craig;Bach JeanMarie;Bachurski Daniel;Baharvand Hossein;Balaj Leonora;Baldacchino Shawn;Bauer Natalie N;Baxter Amy A;Bebawy Mary;Beckham Carla;Zavec Apolonija Bedina;Benmoussa Abderrahim;Berardi Anna C;Bergese Paolo;Bielska Ewa;Blenkiron Cherie;BobisWozowicz Sylwia;Boilard Eric;Boireau Wilfrid;Bongiovanni Antonella;Borras Francesc E;Bosch Steffi;Boulanger Chantal M;Breakefield Xandra;Breglio Andrew M;Brennan Meadhbh A;Brigstock David R;Brisson Alain;Broekman Marike L D;Bromberg Jacqueline F;BrylGorecka Paulina;Buch Shilpa;Buck Amy H;Burger Dylan;Busatto Sara;Buschmann Dominik;Bussolati Benedetta;Buzas Edit I;Byrd James Bryan;Camussi Giovanni;Carter David R F;Caruso Sarah;Chamley Lawrence W;Chang YuTing;Chen Chihchen;Chen Shuai;Cheng Lesley;Chin Andrew R;Clayton Aled;Clerici Stefano P;Cocks Alex;Cocucci Emanuele;Coffey Robert J;CordeirodaSilva Anabela;Couch Yvonne;Coumans Frank A W;Coyle Beth;Crescitelli Rossella;Criado Miria Ferreira;D'SouzaSchorey Crislyn;Das Saumya;Chaudhuri Amrita Datta;de Candia Paola;De Santana Junior Eliezer F;De Wever Olivier;del Portillo Hernando A;Demaret Tanguy;Deville Sarah;Devitt Andrew;Dhondt Bert;Di Vizio Dolores;Dieterich Lothar C;Dolo Vincenza;Dominguez Rubio Ana Paula;Dominici Massimo;Dourado Mauricio R;Driedonks Tom A P;Duarte Filipe V;Duncan Heather M;Eichenberger Ramon M;Ekstrom Karin;Andaloussi Samir E L;ElieCaille Celine;Erdbrugger Uta;FalconPerez Juan M;Fatima Farah;Fish Jason E;FloresBellver Miguel;Forsonits Andras;FreletBarrand Annie;Fricke Fabia;Fuhrmann Gregor;Gabrielsson Susanne;GamezValero Ana;Gardiner Chris;Gaertner Kathrin;Gaudin Raphael;Gho Yong Song;Giebel Bernd;Gilbert Caroline;Gimona Mario;Giusti Ilaria;Goberdhan Deborah C I;Goergens Andre;Gorski Sharon M;Greening David W;Gross Julia Christina;Gualerzi Alice;Gupta Gopal N;Gustafson Dakota;Handberg Aase;Haraszti Reka A;Harrison Paul;Hegyesi Hargita;Hendrix An;Hill Andrew F;Hochberg Fred H;Hoffmann Karl F;Holder Beth;Holthofer Harry;Hosseinkhani Baharak;Hu Guoku;Huang Yiyao;Huber Veronica;Hunt Stuart;Ibrahim Ahmed GamalEldin;Ikezu Tsuneya;Inal Jameel M;Isin Mustafa;Ivanova Alena;Jackson Hannah K;Jacobsen Soren;Jay Steven M;Jayachandran Muthuvel;Jenster Guido;Jiang Lanzhou;Johnson Suzanne M;Jones Jennifer C;Jong Ambrose;JovanovicTalisman Tijana;Jung Stephanie;Kalluri Raghu;Kano Shinichi;Kaur Sukhbir;Kawamura Yumi;Keller Evan T;Khamari Delaram;Khomyakova Elena;Khvorova Anastasia;Kierulf Peter;Kim Kwang Pyo;Kislinger Thomas;Klingeborn Mikael;Klinke David J II;Kornek Miroslaw;Kosanovic Maja M;Kovacs Arpad Ferenc;KraemerAlbers EvaMaria;Krasemann Susanne;Krause Mirja;Kurochkin Igor V;Kusuma Gina D;Kuypers Soren;Laitinen Saara;Langevin Scott M;Languino Lucia R;Lannigan Joanne;Lasser Cecilia;Laurent Louise C;Lavieu Gregory;LazaroIbanez Elisa;Le Lay Soazig;Lee MyungShin;Lee Yi Xin Fiona;Lemos Debora S;Lenassi Metka;Leszczynska Aleksandra;Li Isaac T S;Liao Ke;Libregts Sten F;Ligeti Erzsebet;Lim Rebecca;Lim Sai Kiang;Line Aija;Linnemannstoens Karen;Llorente Alicia;Lombard Catherine A;Lorenowicz Magdalena J;Lorincz Akos M;Lotvall Jan;Lovett Jason;Lowry Michelle C;Loyer Xavier;Lu Quan;Lukomska Barbara;Lunavat Taral R;Maas Sybren L N;Malhi Harmeet;Marcilla Antonio;Mariani Jacopo;Mariscal Javier;MartensUzunova Elena S;MartinJaular Lorena;Martinez M Carmen;Martins Vilma Regina;Mathieu Mathilde;Mathivanan Suresh;Maugeri Marco;McGinnis Lynda K;McVey Mark J;Meckes David G Jr;Meehan Katie L;Mertens Inge;Minciacchi Valentina R;Moller Andreas;Jorgensen Malene Moller;MoralesKastresana Aizea;Morhayim Jess;Mullier Francois;Muraca Maurizio;Musante Luca;Mussack Veronika;Muth Dillon C;Myburgh Kathryn H;Najrana Tanbir;Nawaz Muhammad;Nazarenko Irina;Nejsum Peter;Neri Christian;Neri Tommaso;Nieuwland Rienk;Nimrichter Leonardo;Nolan John P;Nolte't Hoen Esther N M;Noren Hooten 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M;Skowronek Agata;Snyder Orman L II;Soares Rodrigo Pedro;Sodar Barbara W;Soekmadji Carolina;Sotillo Javier;Stahl Philip D;Stoorvogel Willem;Stott Shannon L;Strasser Erwin F;Swift Simon;Tahara Hidetoshi;Tewari Muneesh;Timms Kate;Tiwari Swasti;Tixeira Rochelle;Tkach Mercedes;Toh Wei Seong;Tomasini Richard;Torrecilhas Ana Claudia;Pablo Tosar Juan;Toxavidis Vasilis;Urbanelli Lorena;Vader Pieter;van Balkom Bas W M;van der Grein Susanne G;Van Deun Jan;van Herwijnen Martijn J C;Van KeurenJensen Kendall;van Niel Guillaume;van Royen Martin E;van Wijnen Andre J;Helena Vasconcelos M;Vechetti Ivan J Jr;Veit Tiago D;Vella Laura J;Velot Emilie;Verweij Frederik J;Vestad Beate;Vinas Jose L;Visnovitz Tamas;Vukman Krisztina V;Wahlgren Jessica;Watson Dionysios C;Wauben Marca H M;Weaver Alissa;Webber Jason P;Weber Viktoria;Wehman Ann M;Weiss Daniel J;Welsh Joshua A;Wendt Sebastian;Wheelock Asa M;Wiener Zoltan;Witte Leonie;Wolfram Joy;Xagorari Angeliki;Xander Patricia;Xu Jing;Yan Xiaomei;YanezMo Maria;Yin Hang;Yuana Yuana;Zappulli Valentina;Zarubova Jana;Zekas Vytautas;Zhang Jianye;Zhao Zezhou;Zheng Lei;Zheutlin Alexander R;Zickler Antje M;Zimmermann Pascale;Zivkovic Angela M;Zocco Davide;ZubaSurma Ewa K
2018
Abstract
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/385910
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Il report seguente simula gli indicatori relativi alla propria produzione scientifica in relazione alle soglie ASN 2023-2025 del proprio SC/SSD. Si ricorda che il superamento dei valori soglia (almeno 2 su 3) è requisito necessario ma non sufficiente al conseguimento dell'abilitazione. La simulazione si basa sui dati IRIS e sugli indicatori bibliometrici alla data indicata e non tiene conto di eventuali periodi di congedo obbligatorio, che in sede di domanda ASN danno diritto a incrementi percentuali dei valori. La simulazione può differire dall'esito di un’eventuale domanda ASN sia per errori di catalogazione e/o dati mancanti in IRIS, sia per la variabilità dei dati bibliometrici nel tempo. Si consideri che Anvur calcola i valori degli indicatori all'ultima data utile per la presentazione delle domande.
La presente simulazione è stata realizzata sulla base delle specifiche raccolte sul tavolo ER del Focus Group IRIS coordinato dall'Università di Modena e Reggio Emilia e delle regole riportate nel DM 589/2018 e allegata Tabella A. Cineca, l'Università di Modena e Reggio Emilia e il Focus Group IRIS non si assumono alcuna responsabilità in merito all’uso che il diretto interessato o terzi faranno della simulazione. Si specifica inoltre che la simulazione contiene calcoli effettuati con dati e algoritmi di pubblico dominio e deve quindi essere considerata come un mero ausilio al calcolo svolgibile manualmente o con strumenti equivalenti.