Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles.

Outer membrane vesicles (OMVs) are potent virulence factors, naturally secreted by gram-negative bacteria.Since Klebsiella pneumoniae has emerged as an important nosocomial pathogen, because of resistance to a widespectrum of antibiotics, it is crucial to investigate its pathogenetic mechanism microorganism secretes outermembrane vesicles (OMVs), but the pathogenesis of Klebsiella pneumoniae as it relates to OMVs has not been wellelucidated. In this study we focused on the isolation, characterization and evaluation of the virulence potential ofOMVs obtained from Klebsiella pneumoniae. Our data demonstrate that Klebsiella pneumoniae OMVs are importantsecretory nanocomplexes that elicit a potent inflammatory response. Since OMVs are clearly involved in thepathogenesis of this bacterium during infection, further studies are required to determine whether they could befuture targets for novel therapy and potential vaccine against Klebsiella pneumoniae.