In the past few years, extensive research has been done to develop proper drug delivery platforms that can deliver therapeutic agents to specific sites of the body or targeted cells, minimizing side effects. Similar to the microenvironment of soft tissues, hydrogels are a novel type of delivery platform with a 3D interconnected structure formed by physically or chemically cross-linked polymer networks. Drugs can be incorporated in the tridimensional scaffold and successfully vehiculated/administered into specific sites of the organism1. Hyaluronic acid (HA) is a high molecular weight negatively charged polysaccharide composed of repeating disaccharide units, which is abundant in the synovial fluid and extracellular matrix, but has also been found intracellularly. HA is involved in several biological processes such as the maintenance of the viscoelasticity of connective fluids, tissue hydration, morphogenesis and cell repair2. For these reasons it represents a valid biomaterial for tissue engineering as component of hydrogel platforms, thanks further to its good biocompatibility, biodegradability and non-immunogenicity. Currently, we are exploring the best experimental conditions to obtain a hydrogel scaffold formed by the interactions between an amphiphilic network (HA-TDA) and a cationic cyclodextrin polymer (Poly-CD). Once formed, the construct can potentially incorporate various therapeutic cargos (i.e. drugs, growth factors, exosomes, cell adhesion promoters) and diagnostic probes (i.e. fluorophores and magnetic nanoparticles), to be used as cell-laden material for bioprinting in regenerative medicine applications3. HA-TDA was synthesized by coupling of hydrophobic tetradecylamine (TDA) to hyaluronic acid (HA) in the presence of 2-chloro-4,6-dimethoxy-2,3,5-triazine (CDMT) and N-methylmorpholine (NMM). Complementary spectroscopic techniques such as UV-Vis, steady-state and time-resolved fluorescence, DLS and z-potential measurement have been used to investigate the interactions and to monitor the assembly between HA-TDA and Poly-CD at different mass ratios and the entrapment capability of HA@CD assembly.
HYDROGELS BASED ON HYALURONIC ACID/CYCLODEXTRIN ASSEMBLIES FOR REGENERATIVE MEDICINE
Annalaura Cordaro;Roberto Zagami;Antonino Mazzaglia
2018
Abstract
In the past few years, extensive research has been done to develop proper drug delivery platforms that can deliver therapeutic agents to specific sites of the body or targeted cells, minimizing side effects. Similar to the microenvironment of soft tissues, hydrogels are a novel type of delivery platform with a 3D interconnected structure formed by physically or chemically cross-linked polymer networks. Drugs can be incorporated in the tridimensional scaffold and successfully vehiculated/administered into specific sites of the organism1. Hyaluronic acid (HA) is a high molecular weight negatively charged polysaccharide composed of repeating disaccharide units, which is abundant in the synovial fluid and extracellular matrix, but has also been found intracellularly. HA is involved in several biological processes such as the maintenance of the viscoelasticity of connective fluids, tissue hydration, morphogenesis and cell repair2. For these reasons it represents a valid biomaterial for tissue engineering as component of hydrogel platforms, thanks further to its good biocompatibility, biodegradability and non-immunogenicity. Currently, we are exploring the best experimental conditions to obtain a hydrogel scaffold formed by the interactions between an amphiphilic network (HA-TDA) and a cationic cyclodextrin polymer (Poly-CD). Once formed, the construct can potentially incorporate various therapeutic cargos (i.e. drugs, growth factors, exosomes, cell adhesion promoters) and diagnostic probes (i.e. fluorophores and magnetic nanoparticles), to be used as cell-laden material for bioprinting in regenerative medicine applications3. HA-TDA was synthesized by coupling of hydrophobic tetradecylamine (TDA) to hyaluronic acid (HA) in the presence of 2-chloro-4,6-dimethoxy-2,3,5-triazine (CDMT) and N-methylmorpholine (NMM). Complementary spectroscopic techniques such as UV-Vis, steady-state and time-resolved fluorescence, DLS and z-potential measurement have been used to investigate the interactions and to monitor the assembly between HA-TDA and Poly-CD at different mass ratios and the entrapment capability of HA@CD assembly.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
