Osteoarthritis (OA) is a prevalent, severe degenerative disease that affects about 50% of the over-sixty population. While various therapeutic (pharmacological, surgical) options are available in clinics to manage the progression of OA, none of them are able to reproduce the original hyaline articular cartilage in affected patients thus, there is an urgent need for new, improved treatments for OA. Intra-articular delivery of anti-inflammatory agents that can facilitate the regeneration processes is currently a challenging area of research that requires the use of biocompatible and non-immunogenic carriers1. Hyaluronic acid (HA) is used to promote chondroprotection, shock absorption, and anti-inflammatory effects2, but it is rapidly cleared from the joint area. Here, we develope a more structured material based on cationic cyclodextrin polymer (PolyCD) and HA covalently linked to ?-cyclodextrin. The supramolecular nanoassembly is a tridimensional interconnected network obtained by the bifunctional adamantanyl cross-linker agent (Bis-Ada). The nanocarrier was loaded with the fluorescent probe Ada-Rhod and with the anti-inflammatory agent Diclofenac (DCF). Complementary spectroscopic techniques including 1H-NMR, UV-Vis, steady-state and time-resolved fluorescence, DLS and z-potential measurement have been used to investigate the interactions and to monitor the assembly formation and entrapment capability. All our evidences allowed elucidating physico-chemical properties of nanoassemblies in ultrapure water and in biological relevant media for further investigation in vitro OA models.
CYCLODEXTRIN-BASED NANOASSEMBLIES AS THERAPEUTIC SCAFFOLDS FOR THE TREATMENT OF INFLAMMATORY DISEASES
Annalaura Cordaro;Roberto Zagami;Antonino Mazzaglia
2019
Abstract
Osteoarthritis (OA) is a prevalent, severe degenerative disease that affects about 50% of the over-sixty population. While various therapeutic (pharmacological, surgical) options are available in clinics to manage the progression of OA, none of them are able to reproduce the original hyaline articular cartilage in affected patients thus, there is an urgent need for new, improved treatments for OA. Intra-articular delivery of anti-inflammatory agents that can facilitate the regeneration processes is currently a challenging area of research that requires the use of biocompatible and non-immunogenic carriers1. Hyaluronic acid (HA) is used to promote chondroprotection, shock absorption, and anti-inflammatory effects2, but it is rapidly cleared from the joint area. Here, we develope a more structured material based on cationic cyclodextrin polymer (PolyCD) and HA covalently linked to ?-cyclodextrin. The supramolecular nanoassembly is a tridimensional interconnected network obtained by the bifunctional adamantanyl cross-linker agent (Bis-Ada). The nanocarrier was loaded with the fluorescent probe Ada-Rhod and with the anti-inflammatory agent Diclofenac (DCF). Complementary spectroscopic techniques including 1H-NMR, UV-Vis, steady-state and time-resolved fluorescence, DLS and z-potential measurement have been used to investigate the interactions and to monitor the assembly formation and entrapment capability. All our evidences allowed elucidating physico-chemical properties of nanoassemblies in ultrapure water and in biological relevant media for further investigation in vitro OA models.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
