Cardoon (Cynara cardunculus L. var. altilis) is a traditional Italian vegetable crop, with high yields of seed oil with a good fatty acid profile (high oleic acid) and a vigorous lignocellulosic biomass characterized by valuable compounds that can be recovered both from the apical part (e.g. chlorogenic acid and other polyphenols) and from roots (inulin). To overcome the hurdles of seasonal availability and high biochemical variability of the biomass, standardized cardoon cell cultures represent an alternative to field cultivation. In this frame, we tested a biotechnological approach via Agrobacterium-mediated transformation to obtain stable transgenic cardoon cell lines with the aim of increasing the accessibility of the cellulose fraction and of boosting the production of high-value nutraceuticals. Our strategy revolved around the overexpression of the Arabidopsis MYB4 gene, encoding a transcriptional factor known to be a master regulator of the phenylpropanoid pathway. We provide a detailed characterization of the transgenic lines based on molecular, metabolic and transcriptomic comparisons with wild-type controls. The research activities here described are part of the larger project BOBCAt (funded by Cariplo) devoted to optimize and scale-up the use of cardoon cell cultures in economically and environmentally sustainable conditions, in line with the principles of Circular Economy.
Agrobacterium-mediated transformation of cardoon cell cultures allows alteration of the phenylpropanoid pathway
Paolo D;Locatelli F;Cominelli E;Pirona R;Docimo T;Tucci M;Sparvoli F
2021
Abstract
Cardoon (Cynara cardunculus L. var. altilis) is a traditional Italian vegetable crop, with high yields of seed oil with a good fatty acid profile (high oleic acid) and a vigorous lignocellulosic biomass characterized by valuable compounds that can be recovered both from the apical part (e.g. chlorogenic acid and other polyphenols) and from roots (inulin). To overcome the hurdles of seasonal availability and high biochemical variability of the biomass, standardized cardoon cell cultures represent an alternative to field cultivation. In this frame, we tested a biotechnological approach via Agrobacterium-mediated transformation to obtain stable transgenic cardoon cell lines with the aim of increasing the accessibility of the cellulose fraction and of boosting the production of high-value nutraceuticals. Our strategy revolved around the overexpression of the Arabidopsis MYB4 gene, encoding a transcriptional factor known to be a master regulator of the phenylpropanoid pathway. We provide a detailed characterization of the transgenic lines based on molecular, metabolic and transcriptomic comparisons with wild-type controls. The research activities here described are part of the larger project BOBCAt (funded by Cariplo) devoted to optimize and scale-up the use of cardoon cell cultures in economically and environmentally sustainable conditions, in line with the principles of Circular Economy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
