Dna damage in human amniotic cells: Antigenotoxic potential of curcumin and alpha-lipoic acid

Oxidative imbalances in the gestational phase are responsible for certain complications during pregnancy and for foetal and neonatal genetic disorders. In this work, using human amnio-cytes, we aimed to evaluate the protection provided to foetal DNA by two concentrations of anti-oxidant molecules, ?-lipoic acid (LA) and curcumin (Cur), against hydrogen peroxide (H2O2)-in-duced damage. Genotoxicity tests, performed by the random amplification of polymorphic DNA (RAPD-PCR) technique and TUNEL tests, showed that the lowest concentration of LA-protected cells and DNA from H2O2 insults. However, a greater ability to protect the amniocytes' DNA against H2O2 was observed following co-treatment with the highest concentration of Cur with H2O2. In fact, a genomic template stability (GTS%) similar to that of the negative control and a statistically signif-icant reduction in the DNA fragmentation index (DFI) were revealed. Moreover, following a com-bined treatment with both antioxidants and H2O2, no statistical difference from controls was ob-served, in terms of both induced mutations and DNA breaks. Furthermore, no effect on morphology or cell viability was observed. The results demonstrate the ability of LA and Cur to protect the genetic material of amniocytes against genotoxic insults, suggesting their beneficial effects in pathol-ogies related to oxidative stress.