Cardoon (Cynara cardunculus L. var. altilis) is a traditional crop with high yields of seed oil with a good fatty acid profile and a vigorous lignocellulosic biomass characterized by valuable phenolic compounds (e.g. chlorogenic acid and other polyphenols) (1). To overcome the hurdles of seasonal availability and high biochemical variability of the biomass, standardized cardoon cell cultures represent an alternative to field cultivation (2). We tested a biotechnological approach via Agrobacterium-mediated transformation, to obtain stable transgenic cardoon cell lines overexpressing the Arabidopsis MYB4 gene, encoding a transcription factor known to be a master regulator of the phenylpropanoid pathway (3)
Agrobacterium-mediated transformation of cardoon cell cultures allows alteration of the phenylpropanoid pathway.
Paolo D;Locatelli F;Cominelli E;Docimo T;Tucci M;Sparvoli F
2021
Abstract
Cardoon (Cynara cardunculus L. var. altilis) is a traditional crop with high yields of seed oil with a good fatty acid profile and a vigorous lignocellulosic biomass characterized by valuable phenolic compounds (e.g. chlorogenic acid and other polyphenols) (1). To overcome the hurdles of seasonal availability and high biochemical variability of the biomass, standardized cardoon cell cultures represent an alternative to field cultivation (2). We tested a biotechnological approach via Agrobacterium-mediated transformation, to obtain stable transgenic cardoon cell lines overexpressing the Arabidopsis MYB4 gene, encoding a transcription factor known to be a master regulator of the phenylpropanoid pathway (3)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
