Pinus nigra J.F. Arnold has an ecological and economic interest in Europe, but many of the planted populations have an unknown origin and infraspecific taxonomy. Six Portuguese P. nigra populations characterised with microsatellites revealed high intra-population diversity structured into two clusters with low differentiation that might suggest two provenances or infraspecific taxa. Despite compared with foreign samples from different subspecies, we were not able to infer about the origin or infraspecific taxonomy of the Portuguese populations based on the pooled microsatellite data .
Portuguese Pinus nigra JF Arnold populations: genetic diversity, structure and relationships inferred by SSR markers
Ilaria Spanu;Giovanni Giuseppe Vendramin;Francesca Bagnoli;
2020
Abstract
Pinus nigra J.F. Arnold has an ecological and economic interest in Europe, but many of the planted populations have an unknown origin and infraspecific taxonomy. Six Portuguese P. nigra populations characterised with microsatellites revealed high intra-population diversity structured into two clusters with low differentiation that might suggest two provenances or infraspecific taxa. Despite compared with foreign samples from different subspecies, we were not able to infer about the origin or infraspecific taxonomy of the Portuguese populations based on the pooled microsatellite data .File in questo prodotto:
| File | Dimensione | Formato | |
|---|---|---|---|
|
Dias_et_al_AoFS,_2020.pdf
accesso aperto
Descrizione: Pinus nigraJ.F. Arnold has an ecological ande conomic interest in Europe,but many of the planted populations have an unknown origin and infraspecific taxonomy. Six Portuguese P. nigra populations characterised with microsatellites revealed high intra-population diversity structured into two clusters with low differentiation that might suggest two provenances or infraspecific taxa. Despite compared with foreign samples from different subspecies, we were not able to infer about the origin or infraspecific taxonomy of the Portuguese populations based on the pooled microsatellite data. & Context Many ofthe European Pinus nigra J.F. Arnold forests were planted with the material of unknown origin. Efforts have been madetodetermine their provenances and infraspecific taxonomy regarding their relevance for defining strategies of genetic resources conservation, germplasm use, forest management and genetic improvement. The Portuguese P. nigra populations are allochthonous, and their provenance and infraspecific taxonomy are unknown. & Aims With this work, we intended to characterise the Portuguese P. nigra populations regarding its genetic diversity, structure and relationships using simple sequence repeat (SSR) markers and/or to infer about its origin and infraspecific taxonomy by comparing the SSR patterns of foreign P. nigra samples of known taxonomic classification. & Methods A total of 224 Portuguese P. nigra individuals from six populations were characterised using 14 SSR markers developed specifically to different Pinus sp., including P. nigra, by other authors. & Results Thirteen SSR loci were selected and showed 100% of polymorphism among individuals. The genetic diversity was higher within (95%) rather than among (5%)populations. The Portugueseindividuals were structured intotwo mainclusters(K = 2) withlowgenetic differentiation (FST = 0.04), and the foreign samples weregenetically distant from the Portugueseindividuals. & Conclusion The six Portuguese P. nigra populations revealed high genetic diversity and seemed to be structured into two main clusters with low differentiation suggesting two provenances or infraspecific taxa. Nonetheless, the comparative analyses with foreign samples did not allow a clear inference about its origin and/or infraspecific taxonomy. Additional foreign samples and/or molecular markers will be tested to pursue these goals.
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
909.67 kB
Formato
Adobe PDF
|
909.67 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
