Long-Term Changes in the Zooplankton Community of Lake Maggiore in Response to Multiple Stressors: A Functional Principal Components Analysis

We describe the long-term (1981-2008) dynamics of several physico-chemical and biological variables and how their changes may have influenced zooplankton structure in Lake Maggiore (Italy). Data was available for the 1981-1992 and 1995-2008 periods. Standardized time-series for temperature and total phosphorus (TP), chlorophyll-a, phytoplankton density (cel m(-3)), and cell size (mu m(3)), as well as zooplankton structure (Copepoda, Cladocera, and Rotifera density, ind m(-3)) were smoothed using penalized B-splines and analyzed using Functional Principal Components (FPCs) to assess their dominant modes of variation. The first four FPCs explained 55% of 1981-1992 and 65% of 1995-2008 overall variation. Results showed that temperature fluctuated during the study period, particularly during 1988-1992 with a general tendency to increase. TP showed a declining trend with some reversions in the pattern observed in the years 1992, 1999, and 2000. Phytoplankton estimators and chlorophyll-a concentration showed a variable trend along the study period. Zooplankton groups also had a variable trend along the study period with a general increase in density of large carnivorous (mainly Bythotrephes longimanus) and a decrease of large herbivorous (mainly Daphnia), and a similar increase in the ratio of raptorial to microphagous rotifers. Our results suggest that the lake experienced a strong trophic change associated with oligotrophication, followed by pronounced climate-induced changes during the latter period. TP concentration was strongly associated with changes in abundance of some zooplankton taxa.