first_pagesettings Open AccessArticle Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat by Francesca Taranto 1OrcID,Nunzio D'Agostino 2OrcID,Marcello Catellani 3,+,Luca Laviano 3,?,Domenico Ronga 3,§,Justyna Milc 3OrcID,Barbara Prandi 4OrcID,Fatma Boukid 4,?OrcID,Stefano Sforza 4,Sara Graziano 5,Mariolina Gullì 5OrcID,Giovanna Visioli 5OrcID,Nelson Marmiroli 5,Franz-W. Badeck 6OrcID,Anna Paola Minervini 7,Ivano Pecorella 7,Nicola Pecchioni 7,Pasquale De Vita 7OrcID andEnrico Francia 3,*OrcID 1 Institute of Biosciences and Bioresources (CNR-IBBR), Portici, 80055 Naples, Italy 2 Department of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, Italy 3 Department of Life Sciences, Centre BIOGEST-SITEIA, University of Modena and Reggio Emilia, 42122 Reggio Emilia, Italy 4 Department of Food and Drug, Centre SITEIA.PARMA, University of Parma, 43124 Parma, Italy 5 Department of Chemistry, Life Sciences and Environmental Sustainability, Centre SITEIA.PARMA, University of Parma, 43124 Parma, Italy 6 Genomics and Bioinformatics Research Centre, CREA-GB, 29017 Fiorenzuola d'Arda (Piacenza), Italy 7 Research Centre for Cereal and Industrial Crops, CREA-CI, 71122 Foggia, Italy * Author to whom correspondence should be addressed. + Present address: Department for Sustainability, CR ENEA-Trisaia, 75026 Rotondella (Matera), Italy. ? Present address: Rijk Zwaan B.V., 2678 KX De Lier (Westland), The Netherlands. § Present address: Centro Ricerche Produzioni Animali-CRPA S.p.A, 42124 Reggio Emilia, Italy. ? Present address: IRTA Institute of Agrifood Research and Technology, 17121 Monells (Girona), Spain. Agronomy 2020, 10(9), 1231; https://doi.org/10.3390/agronomy10091231 Received: 10 July 2020 / Revised: 6 August 2020 / Accepted: 19 August 2020 / Published: 20 August 2020 (This article belongs to the Section Crop Breeding and Genetics) Download PDF Browse Figures Review Reports Abstract While durum wheat is a major food source in Mediterranean countries, storage (i.e., gluten) proteins are however responsible for celiac disease (CD), a serious autoimmune disease that occurs in genetically predisposed subjects. Different gluten epitopes--defined as "immunogenic" (IP) and "toxic" (TP) peptides--are involved in the pathology and their content in wheat grain depends on environmental and genetic factors. Detection of IP and TP is not trivial, and no work has been conducted so far to identify the genomic regions associated with their accumulation in wheat. In the present study, a genome-wide association study was performed on a durum wheat collection to identify marker-trait associations (MTAs) between 5730 high quality SNPs and the accumulation of CD-related peptides and gluten protein composition measured in two consecutive cropping seasons (2015/2016 and 2016/2017). High-molecular-weight glutenin subunits (HMW-GS) were more stable between the two years, and differences in total gluten proteins were mainly due to low-molecular-weight glutenin subunits (LMW-GS) and accumulation of gliadins. In the first instance, association tests were conducted on yellow pigment content (YP), a highly inheritable trait with a well-known genetic basis, and several significant MTAs were found corresponding to loci already known for being related to YP. These findings showed that MTAs found for the rest of the measured traits were reliable. In total, 28 significant MTAs were found for gluten composition, while 14 were found to be associated with IP and TP. Noteworthy, neither significant (-log10p > 4.7) nor suggestive (-log10p > 3.3) MTAs for the accumulation of CD-triggering epitopes were found on Gli-A1/Glu-A3 and Gli-B1/Glu-B3 loci, thus suggesting regulatory rather than structural gene effect. A PBF transcription factor on chromosome 5B, known to be involved in the regulation of the expression of CD-related peptides, was identified among the positional candidate genes in the LD-decay range around significant SNPs. Results obtained in the present study provide useful insights and resources for the long-term objective of selecting low-toxic durum wheat varieties while maintaining satisfactory gluten quality.
Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat
Taranto F;
2020
Abstract
first_pagesettings Open AccessArticle Characterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheat by Francesca Taranto 1OrcID,Nunzio D'Agostino 2OrcID,Marcello Catellani 3,+,Luca Laviano 3,?,Domenico Ronga 3,§,Justyna Milc 3OrcID,Barbara Prandi 4OrcID,Fatma Boukid 4,?OrcID,Stefano Sforza 4,Sara Graziano 5,Mariolina Gullì 5OrcID,Giovanna Visioli 5OrcID,Nelson Marmiroli 5,Franz-W. Badeck 6OrcID,Anna Paola Minervini 7,Ivano Pecorella 7,Nicola Pecchioni 7,Pasquale De Vita 7OrcID andEnrico Francia 3,*OrcID 1 Institute of Biosciences and Bioresources (CNR-IBBR), Portici, 80055 Naples, Italy 2 Department of Agricultural Sciences, University of Naples Federico II, Portici, 80055 Naples, Italy 3 Department of Life Sciences, Centre BIOGEST-SITEIA, University of Modena and Reggio Emilia, 42122 Reggio Emilia, Italy 4 Department of Food and Drug, Centre SITEIA.PARMA, University of Parma, 43124 Parma, Italy 5 Department of Chemistry, Life Sciences and Environmental Sustainability, Centre SITEIA.PARMA, University of Parma, 43124 Parma, Italy 6 Genomics and Bioinformatics Research Centre, CREA-GB, 29017 Fiorenzuola d'Arda (Piacenza), Italy 7 Research Centre for Cereal and Industrial Crops, CREA-CI, 71122 Foggia, Italy * Author to whom correspondence should be addressed. + Present address: Department for Sustainability, CR ENEA-Trisaia, 75026 Rotondella (Matera), Italy. ? Present address: Rijk Zwaan B.V., 2678 KX De Lier (Westland), The Netherlands. § Present address: Centro Ricerche Produzioni Animali-CRPA S.p.A, 42124 Reggio Emilia, Italy. ? Present address: IRTA Institute of Agrifood Research and Technology, 17121 Monells (Girona), Spain. Agronomy 2020, 10(9), 1231; https://doi.org/10.3390/agronomy10091231 Received: 10 July 2020 / Revised: 6 August 2020 / Accepted: 19 August 2020 / Published: 20 August 2020 (This article belongs to the Section Crop Breeding and Genetics) Download PDF Browse Figures Review Reports Abstract While durum wheat is a major food source in Mediterranean countries, storage (i.e., gluten) proteins are however responsible for celiac disease (CD), a serious autoimmune disease that occurs in genetically predisposed subjects. Different gluten epitopes--defined as "immunogenic" (IP) and "toxic" (TP) peptides--are involved in the pathology and their content in wheat grain depends on environmental and genetic factors. Detection of IP and TP is not trivial, and no work has been conducted so far to identify the genomic regions associated with their accumulation in wheat. In the present study, a genome-wide association study was performed on a durum wheat collection to identify marker-trait associations (MTAs) between 5730 high quality SNPs and the accumulation of CD-related peptides and gluten protein composition measured in two consecutive cropping seasons (2015/2016 and 2016/2017). High-molecular-weight glutenin subunits (HMW-GS) were more stable between the two years, and differences in total gluten proteins were mainly due to low-molecular-weight glutenin subunits (LMW-GS) and accumulation of gliadins. In the first instance, association tests were conducted on yellow pigment content (YP), a highly inheritable trait with a well-known genetic basis, and several significant MTAs were found corresponding to loci already known for being related to YP. These findings showed that MTAs found for the rest of the measured traits were reliable. In total, 28 significant MTAs were found for gluten composition, while 14 were found to be associated with IP and TP. Noteworthy, neither significant (-log10p > 4.7) nor suggestive (-log10p > 3.3) MTAs for the accumulation of CD-triggering epitopes were found on Gli-A1/Glu-A3 and Gli-B1/Glu-B3 loci, thus suggesting regulatory rather than structural gene effect. A PBF transcription factor on chromosome 5B, known to be involved in the regulation of the expression of CD-related peptides, was identified among the positional candidate genes in the LD-decay range around significant SNPs. Results obtained in the present study provide useful insights and resources for the long-term objective of selecting low-toxic durum wheat varieties while maintaining satisfactory gluten quality.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
