Corylus avellana L. is known for its healthy properties, however previous studies have not dealt with its effects on human innate response. The aim of this study is to assess if C. avellana has an immune adjuvant effect. Human macrophages were pre-treated with hazelnut liquid extract, found to be rich in phenylpropanoids, and then infected with Staphylococcus aureus. Based on the intracellular bacteria containment rate and on the treatment doses used, the donors were divided into High-Dose and Low-Dose-Responders. Expression profile for inflammatory and Iron metabolism genes, both for Peripheral Blood Mononuclear Cells and Macrophages, highlighted that Low-Dose-Responders came from a more pronounced pro-inflammatory milieu as compared with High-Dose-Responders. Laboratory blood test results revealed that Low-Dose-Responders had higher LDL C and Triglycerides and lower HDL C. Overall, our results suggest that the effect of the extract on Macrophage immune response is influenced by the intracellular inflammatory status of the donor.
BIOSERVICE AVANBIO. ENEA- Regione Lazio. F. Mariani. "Analisi del potere immunomodulante della nocciola: l'effetto ormetico su colture primarie di macrofagi umani".
Mariani Francesca
2018
Abstract
Corylus avellana L. is known for its healthy properties, however previous studies have not dealt with its effects on human innate response. The aim of this study is to assess if C. avellana has an immune adjuvant effect. Human macrophages were pre-treated with hazelnut liquid extract, found to be rich in phenylpropanoids, and then infected with Staphylococcus aureus. Based on the intracellular bacteria containment rate and on the treatment doses used, the donors were divided into High-Dose and Low-Dose-Responders. Expression profile for inflammatory and Iron metabolism genes, both for Peripheral Blood Mononuclear Cells and Macrophages, highlighted that Low-Dose-Responders came from a more pronounced pro-inflammatory milieu as compared with High-Dose-Responders. Laboratory blood test results revealed that Low-Dose-Responders had higher LDL C and Triglycerides and lower HDL C. Overall, our results suggest that the effect of the extract on Macrophage immune response is influenced by the intracellular inflammatory status of the donor.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
