Myocytes make up about 75% of ventricular mass but are outnumbered by nonmyocardial cells. Chamber dilatation in progression to heart failure is largely due to myocyte lengthening from series addition of new sarcomeres, while increased cross-sectional area accounts for changes in wall thickness. Coronary perfusion with collagenase can be used to obtain isolated myocytes for determination of myocyte dimensions. It consists of the following steps: (1) heart removal and aortic cannulation; (2) coronary perfusion with media followed by collagenase; (3) myocyte dissociation; (4) myocyte fixation; (5) determination of cell volume (V) by Coulter Channelyzer, length (L) microscopically, and cross-sectional area from V/L. Not only does this approach provide precise, comprehensive, and reproducible results, it allows direct comparisons between all published data collected in this manner.
Assessment of cardiomyocyte size
Pingitore Alessandro
2014
Abstract
Myocytes make up about 75% of ventricular mass but are outnumbered by nonmyocardial cells. Chamber dilatation in progression to heart failure is largely due to myocyte lengthening from series addition of new sarcomeres, while increased cross-sectional area accounts for changes in wall thickness. Coronary perfusion with collagenase can be used to obtain isolated myocytes for determination of myocyte dimensions. It consists of the following steps: (1) heart removal and aortic cannulation; (2) coronary perfusion with media followed by collagenase; (3) myocyte dissociation; (4) myocyte fixation; (5) determination of cell volume (V) by Coulter Channelyzer, length (L) microscopically, and cross-sectional area from V/L. Not only does this approach provide precise, comprehensive, and reproducible results, it allows direct comparisons between all published data collected in this manner.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
