Graphene-Like Layers from Carbon Black: In Vivo Toxicity Assessment

Graphene-like (GL) layers, a new graphene-related material (GRM), possess peculiarchemical, colloidal, optical and transport properties. Considering the very recent promisingapplication of GL layers in biomedical and bioelectronic fields, it is of utmost importance toinvestigate the toxicological profile of these nanomaterials. This study represents an important firstreport of a complete in vivo toxicity assessment of GL layers on embryonic zebrafish (Danio rerio).Our results show that GL layers do not lead to any perturbations in the dierent biological parametersevaluated, indicating their good biocompatibility on a vertebrate model. The new insight into thebiosafety of GL layers will expand their applications in nanomedicine.