Polyhydroxyalkanoates (PHAs) are a family of biopolyesters synthesized by various mi- croorganisms. Due to their biocompatibility and biodegradation, PHAs have been proposed for bi- omedical applications, including tissue engineering scaffolds. Olive leaf extract (OLE) can be ob- tained from agri-food biowaste and is a source of polyphenols with remarkable antioxidant prop- erties. This study aimed at incorporating OLE inside poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) fibers via electrospinning to obtain bioactive bio-based blends that are useful in wound healing. PHBHV/OLE electrospun fibers with a size of 1.29 ± 0.34 ?m were obtained. Fourier trans- form infrared chemical analysis showed a uniform surface distribution of hydrophilic -OH groups, confirming the presence of OLE in the electrospun fibers. The main OLE phenols were released from the fibers within 6 days. The biodegradation of the scaffolds in phosphate buffered saline was in- vestigated, demonstrating an adequate stability in the presence of metalloproteinase 9 (MMP-9), an enzyme produced in chronic wounds. The scaffolds were preliminarily tested in vitro with HFFF2 fibroblasts and HaCaT keratinocytes, suggesting adequate cytocompatibility. PHBHV/OLE fiber meshes hold promising features for wound healing, including the treatment of ulcers, due to the long period of durability in an inflamed tissue environment and adequate cytocompatibility.
Electrospun Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate)/Olive Leaf Extract Fiber Mesh as Prospective Bio-Based Scaffold for Wound Healing
Serena Danti;Niccoletta Barbani;Caterina Cristallini;Andrea Lazzeri
2022
Abstract
Polyhydroxyalkanoates (PHAs) are a family of biopolyesters synthesized by various mi- croorganisms. Due to their biocompatibility and biodegradation, PHAs have been proposed for bi- omedical applications, including tissue engineering scaffolds. Olive leaf extract (OLE) can be ob- tained from agri-food biowaste and is a source of polyphenols with remarkable antioxidant prop- erties. This study aimed at incorporating OLE inside poly(hydroxybutyrate-co-hydroxyvalerate) (PHBHV) fibers via electrospinning to obtain bioactive bio-based blends that are useful in wound healing. PHBHV/OLE electrospun fibers with a size of 1.29 ± 0.34 ?m were obtained. Fourier trans- form infrared chemical analysis showed a uniform surface distribution of hydrophilic -OH groups, confirming the presence of OLE in the electrospun fibers. The main OLE phenols were released from the fibers within 6 days. The biodegradation of the scaffolds in phosphate buffered saline was in- vestigated, demonstrating an adequate stability in the presence of metalloproteinase 9 (MMP-9), an enzyme produced in chronic wounds. The scaffolds were preliminarily tested in vitro with HFFF2 fibroblasts and HaCaT keratinocytes, suggesting adequate cytocompatibility. PHBHV/OLE fiber meshes hold promising features for wound healing, including the treatment of ulcers, due to the long period of durability in an inflamed tissue environment and adequate cytocompatibility.File | Dimensione | Formato | |
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Descrizione: Poly(3-Hydroxybutyrate-Co-3- Hydroxyvalerate)/Olive Leaf Extract Fiber Mesh as Prospective Bio-Based Scaffold for Wound Healing
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