To date, there is no definitive demonstration of the usefulness of positron emission tomography (PET) in studying glucose metabolism in cultured cell lines. Therefore, this study was designed to compare PET with more standardized methods for quantitative assessment of glucose uptake in living untransformed and transformed cells and to validate PET for in vitro metabolic studies. Human colon and breast carcinoma cell lines and mouse embryonic fibroblasts were evaluated for uptake of [(18) F] fludeoxyglucose ([(18) F] FDG) by PET and autoradiography and incorporation of 2- deoxyglucose (2-DG) by colorimetric assay and analyzed for radiotoxic effects of [(18) F] FDG and for the expression levels of glucose transporters. Indeed, [(18) F] Incorporation of FDG on PET was comparable to uptake of [(18) F] FDG by autoradiography and incorporation of 2-DG by colorimetric assay, although radiotracer-based methods exhibited more pronounced differences between individual cell lines.As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. In particular, [(18) F] FDG incorporation led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. although radiotracer-based methods showed more pronounced differences between individual cell lines. As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. In particular, [(18) F] FDG incorporation led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. although radiotracer-based methods showed more pronounced differences between individual cell lines. As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. In particular, [(18) F] FDG incorporation led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. [(18) F] Incorporation of FDG led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. [(18) F] Incorporation of FDG led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET is a valuable and reproducible method for studying tumor cell metabolism in vitro, being representative of differences in the molecular profile of normal and tumor cell lines.
Evalutation of Glucose Uptake in Normal and Cancer Cell Lines by Positron Emission Tomography
Nardelli A;
2015
Abstract
To date, there is no definitive demonstration of the usefulness of positron emission tomography (PET) in studying glucose metabolism in cultured cell lines. Therefore, this study was designed to compare PET with more standardized methods for quantitative assessment of glucose uptake in living untransformed and transformed cells and to validate PET for in vitro metabolic studies. Human colon and breast carcinoma cell lines and mouse embryonic fibroblasts were evaluated for uptake of [(18) F] fludeoxyglucose ([(18) F] FDG) by PET and autoradiography and incorporation of 2- deoxyglucose (2-DG) by colorimetric assay and analyzed for radiotoxic effects of [(18) F] FDG and for the expression levels of glucose transporters. Indeed, [(18) F] Incorporation of FDG on PET was comparable to uptake of [(18) F] FDG by autoradiography and incorporation of 2-DG by colorimetric assay, although radiotracer-based methods exhibited more pronounced differences between individual cell lines.As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. In particular, [(18) F] FDG incorporation led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. although radiotracer-based methods showed more pronounced differences between individual cell lines. As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. In particular, [(18) F] FDG incorporation led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. although radiotracer-based methods showed more pronounced differences between individual cell lines. As expected, these data correlated with glucose transporters 1 to 4 and hexokinase II expression in tumor cell lines and mouse fibroblasts. In particular, [(18) F] FDG incorporation led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. [(18) F] Incorporation of FDG led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET represents a valuable and reproducible method to study tumor cell metabolism in vitro, being representative of the differences in the molecular profile of normal and tumor cell lines. [(18) F] Incorporation of FDG led to low rates of apoptosis, with fibroblasts slightly more sensitive to radiotracer-induced cell death. Quantitative analysis of [(18) F] FDG uptake in living cells by PET is a valuable and reproducible method for studying tumor cell metabolism in vitro, being representative of differences in the molecular profile of normal and tumor cell lines.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
