X-ray induced Photodynamic Therapy (XPDT) is a proposed therapy for deep tumours. The idea is to use the X-ray beam of a standard radiotherapy facility to excite ascintillator which is coupled with a photosensitizing agent which in turn generates reactive oxygen species (ROS) which induce local oxidative stress. Alike instandard Photodynamic Therapy, this oxidative stress may be used to treat tumours. Preliminary results [F. Rossi et al. Sci. Rep. 5 (2015) 7606] demonstrated that canXPDT enhance the efficacy of standard radiotherapy, while reducing its unwanted side effects.This work reports the rationale development of a nanostructure incorporating nanoparticles (NP) for XPDT around a silica core by means of electrostaticadsorption. To this aim, scintillator CeF3 and photosensitizer ZnO, both in form of nanoparticles, have been adsorbed into a polyelectrolyte Layer-by-Layer (LbL)multilayer grown around a SiO2 core. This structure, in a future work, could be a platform for drug delivery.We optimized the growth of the structure basing on results from adsorption on planar substrates, as a function of incubation time, of particle concentration, and ofthe composition of the outer polyelectrolyte layer, having also in mind the need to avoid the formation of micrometric aggregates. This drove the rationale synthesisof the nanocapsules. The resulting structures are studied by Scanning Electron Microscopy, X-ray Microanalysis, Dynamic Light Scattering, and ?-potential analysis.
Rationale design of a layer-by-layer nanostructure for X-ray induced photodynamic therapy
Rossi F;
2020
Abstract
X-ray induced Photodynamic Therapy (XPDT) is a proposed therapy for deep tumours. The idea is to use the X-ray beam of a standard radiotherapy facility to excite ascintillator which is coupled with a photosensitizing agent which in turn generates reactive oxygen species (ROS) which induce local oxidative stress. Alike instandard Photodynamic Therapy, this oxidative stress may be used to treat tumours. Preliminary results [F. Rossi et al. Sci. Rep. 5 (2015) 7606] demonstrated that canXPDT enhance the efficacy of standard radiotherapy, while reducing its unwanted side effects.This work reports the rationale development of a nanostructure incorporating nanoparticles (NP) for XPDT around a silica core by means of electrostaticadsorption. To this aim, scintillator CeF3 and photosensitizer ZnO, both in form of nanoparticles, have been adsorbed into a polyelectrolyte Layer-by-Layer (LbL)multilayer grown around a SiO2 core. This structure, in a future work, could be a platform for drug delivery.We optimized the growth of the structure basing on results from adsorption on planar substrates, as a function of incubation time, of particle concentration, and ofthe composition of the outer polyelectrolyte layer, having also in mind the need to avoid the formation of micrometric aggregates. This drove the rationale synthesisof the nanocapsules. The resulting structures are studied by Scanning Electron Microscopy, X-ray Microanalysis, Dynamic Light Scattering, and ?-potential analysis.File | Dimensione | Formato | |
---|---|---|---|
Rationale design of a layer-by-layer nanostructure for X-ray induced photodynamic therapy.pdf
accesso aperto
Descrizione: Articolo
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
6.4 MB
Formato
Adobe PDF
|
6.4 MB | Adobe PDF | Visualizza/Apri |
Supplementary material.docx
accesso aperto
Descrizione: Appendix A. Supplementary data
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
522.08 kB
Formato
Microsoft Word XML
|
522.08 kB | Microsoft Word XML | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.