Description of Longidorus barsii Radivojevi´c & De Luca sp. n. (Nematoda: Longidoridae) from Serbia and observations on some taxonomic characters

Longidorus barsii sp. n., from Mt Tara in the Balkan Peninsula, is described and characterised by using a polyphasicapproach. The species has numerous males. The female body is 5-7 mm long, rather stout and resembles a large Xiphinema. Thelip region is wide, with rounded sides continuous with the neck, frontally flattened and depressed around the oral aperture, amphidsare pouch-like and distinctly bi-lobed and the odontostyle is moderately long. The nuclei of the pharyngeal glands are in the normalposition, the dorsal nucleus located somewhat posterior to anterior third of bulb. The uteri are long, the distal inner epithelium denselycovered with papilla-like outgrowths. The tail is rounded, bluntly conoid and very short. Alpha-numerical identification codes: A4/5,B45, C3, D3, E2, F3, G 1(2), H1, I2, J1, K67. The morphologically most similar species are L. kheirii, L. polyae and L. profundorum.Additional observations are provided on the anterior body region and genital organs in L. barsii sp. n., L. piceicola, L. silvae, andL.uroshis. Selected features are discussed from the taxonomic and functional points of view. The D2-D3 expansion domains of the 28SrRNA gene and the ITS region of L. barsii sp. n. were amplified and sequenced. Phylogenetic analysis using the D2-D3 expansiondomains of the 28S rRNA gene revealed close evolutionary relationships with L. polyae, L. athesinus and three unidentified Longidorusspp.