Malignant melanoma (MM) is one of the deadliest skin cancers. BRAF mutation testing plays a predominant role in the management of MM patients, because modern targeted therapies essentially consist of inhibitors of BRAF. BRAF V600 mutation must be detected using a FDA-approved (USA) or CE IVD certified (Europe) test. The aim of this study was to compare BRAF mutational testing performed by conventiona lnucleotide sequencing approaches with either real-time PCR (rtPCR )or next-generation sequencing (NGS) assays in a real-life,hospital-based series of advanced MM patients. Consecutive patients fromSardinia, Italy, with histological diagnosis of advanced MM (AJCC stages III Cand IV) and referred a the laboratory of Biomolecular Chemistry (ICB) of the National Research Council (CNR), Sassari, Italy for molecular testing from 2012 through 2019, were enrolled into the study.
Comparison of BRAF testing techniques in patients with advanced malignant melanoma: a real-life study.
Colombino M;Rozzo C;Casula M;Manca A;Palomba G;Pisano M;Palmieri G;
2020
Abstract
Malignant melanoma (MM) is one of the deadliest skin cancers. BRAF mutation testing plays a predominant role in the management of MM patients, because modern targeted therapies essentially consist of inhibitors of BRAF. BRAF V600 mutation must be detected using a FDA-approved (USA) or CE IVD certified (Europe) test. The aim of this study was to compare BRAF mutational testing performed by conventiona lnucleotide sequencing approaches with either real-time PCR (rtPCR )or next-generation sequencing (NGS) assays in a real-life,hospital-based series of advanced MM patients. Consecutive patients fromSardinia, Italy, with histological diagnosis of advanced MM (AJCC stages III Cand IV) and referred a the laboratory of Biomolecular Chemistry (ICB) of the National Research Council (CNR), Sassari, Italy for molecular testing from 2012 through 2019, were enrolled into the study.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
