MicroRNAs (miRNAs) can regulate the expression of potentially every transcript in the cell, and the definition of miRNA-target interactions is crucial to understand their role in all biological processes. However, the identification of the miRNAs that target a specific mRNA remains a challenge. Here, we describe an innovative method called miR-CATCHv2.0 for the high-throughput identification of the miRNA species bound to an RNA of interest. We also describe how this method can overcome the limitations of the current computational and experimental methods available in this field
High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0
Marranci A.Primo
;D'Aurizio R.;Rizzo M.;Poliseno L.
Ultimo
2021
Abstract
MicroRNAs (miRNAs) can regulate the expression of potentially every transcript in the cell, and the definition of miRNA-target interactions is crucial to understand their role in all biological processes. However, the identification of the miRNAs that target a specific mRNA remains a challenge. Here, we describe an innovative method called miR-CATCHv2.0 for the high-throughput identification of the miRNA species bound to an RNA of interest. We also describe how this method can overcome the limitations of the current computational and experimental methods available in this fieldFile in questo prodotto:
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35. Marranci et al., 2021 Melanoma - Methods and Protocols (Methods in Molecular Biology).pdf
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Descrizione: High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0
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