Introduction: Food crops are increasingly susceptible to the challenging impacts ofclimate change, encompassing both abiotic and biotic stresses, that cause yieldlosses. Root-associated microorganisms, including plant growth-promotingbacteria (PGPB), can improve plant growth as well as plant tolerance toenvironmental stresses. The aims of this work were to characterize bacteriaisolated from soil and roots of tomato plants grown in open field.Methods: Biochemical and molecular analyses were used to evaluate the PGPpotential of the considered strains on tomato plants in controlled conditions,also assessing their effects under a water deficit condition. The isolated strainswere classified by 16S gene sequencing and exhibited typical features of PGPB,such as the release of siderophores, the production of proteases, andphosphorous solubilization. Inoculating tomato plants with eleven selectedstrains led to the identification of potentially interesting strains that increasedshoot height and dry weight. Three strains were then selected for the experimentunder water deficit in controlled conditions. The tomato plants were monitoredfrom biometric and physiological point of view, and the effect of inoculation atmolecular level was verified with a targeted RT-qPCR based approach on genesthat play a role under water deficit condition.Results: Results revealed the PGP potential of different bacterial isolates in tomatoplants, both in well-watered and stressed conditions. The used integrated approachallowed to obtain a broader picture of the plant status, from biometric, ecophysiologicaland molecular point of view. Gene expression analysis showed adifferent regulation of genes involved in pathways related to abscisic acid,osmoprotectant compounds and heat shock proteins, depending on the treatments.Discussion: Overall, results showed significant changes in tomato plants due tothe bacterial inoculation, also under water deficit, that hold promise for futurefield applications of these bacterial strains, suggesting that a synergistic andcomplementary interaction between diverse PGPB is an important point to beconsidered for their exploitation.
Diverse plant promoting bacterial species differentially improve tomato plant fitness under water stress
Zampieri E;Giovannini L;Brescia F;Sillo F;Centritto M;Balestrini R
2023
Abstract
Introduction: Food crops are increasingly susceptible to the challenging impacts ofclimate change, encompassing both abiotic and biotic stresses, that cause yieldlosses. Root-associated microorganisms, including plant growth-promotingbacteria (PGPB), can improve plant growth as well as plant tolerance toenvironmental stresses. The aims of this work were to characterize bacteriaisolated from soil and roots of tomato plants grown in open field.Methods: Biochemical and molecular analyses were used to evaluate the PGPpotential of the considered strains on tomato plants in controlled conditions,also assessing their effects under a water deficit condition. The isolated strainswere classified by 16S gene sequencing and exhibited typical features of PGPB,such as the release of siderophores, the production of proteases, andphosphorous solubilization. Inoculating tomato plants with eleven selectedstrains led to the identification of potentially interesting strains that increasedshoot height and dry weight. Three strains were then selected for the experimentunder water deficit in controlled conditions. The tomato plants were monitoredfrom biometric and physiological point of view, and the effect of inoculation atmolecular level was verified with a targeted RT-qPCR based approach on genesthat play a role under water deficit condition.Results: Results revealed the PGP potential of different bacterial isolates in tomatoplants, both in well-watered and stressed conditions. The used integrated approachallowed to obtain a broader picture of the plant status, from biometric, ecophysiologicaland molecular point of view. Gene expression analysis showed adifferent regulation of genes involved in pathways related to abscisic acid,osmoprotectant compounds and heat shock proteins, depending on the treatments.Discussion: Overall, results showed significant changes in tomato plants due tothe bacterial inoculation, also under water deficit, that hold promise for futurefield applications of these bacterial strains, suggesting that a synergistic andcomplementary interaction between diverse PGPB is an important point to beconsidered for their exploitation.File | Dimensione | Formato | |
---|---|---|---|
prod_488977-doc_203519.pdf
accesso aperto
Descrizione: Zampieri_et_al_2023
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
3.08 MB
Formato
Adobe PDF
|
3.08 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.